Bioenergetics drives all biological processes, with cellular metabolism being the central orchestrator of biological function and cell health. Metabolic programs are key indicators of healthy cell function, in addition to being a powerful predictor of cellular performance in in vitro disease models. Metabolic programs can also be tuned in drug discovery, through modulation of metabolic targets, signaling, and substrates—toward reinstating a healthy phenotype, or to optimize and enhance cell performance.
The Agilent Seahorse XF platform measures the two main metabolic pathways, mitochondrial respiration and glycolysis, for live cells in real time, to provide functional kinetic measurements of cellular bioenergetics. The Seahorse XF platform provides the flexibility for researchers to customize experiments to target specific research questions, or to use Agilent Seahorse XF kits and assays for turnkey solutions in a simplified workflow.
The Agilent Mito-rOCR Assay measures mitochondrial respiration in live cells using a fluorescence plate reader. An innovative seal lid creates a semi-closed chamber and produces robust oxygen consumption measurements. A dedicated analysis module within Seahorse Analytics automates data analysis and quickly delivers functional data.
Find out more about how the Seahorse XF platform can help researchers:
Watch the video to find out how tools have progressed the field of cancer and immunotherapy research. This 5-minute educational video discusses how cellular metabolism and Agilent Seahorse XF technology works, and how real-time quantitative and qualitative measurements provide deeper insights into processes including proliferation, differentiation, cell death, and activation — to advance science and develop therapeutic strategies.
Are you interested in learning more about how Agilent Seahorse XF analyzers work? Discover how the key indicators of cellular metabolism, oxygen consumption rate (OCR) and extracellular acidification rate (ECAR), are measured for live cells in real time.
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Interested in cutting-edge tools for mitochondrial research? This video showcases the Agilent Mito-rOCR Assay Kit, which offers an innovative, oil-free approach to measuring mitochondrial respiration on a plate reader. Learn how this kit provides reliable measurements of relative oxygen consumption rate (rOCR), helping researchers detect changes in mitochondrial function with ease.
Discover insights into immune cell processes including activation, proliferation, and memory development through functional real-time metabolic measurements.
Reveal cancer metabolic liabilities through functional bioenergetic analysis of live cells in real time for a deeper understanding of cancer cell biology.
Discover cellular metabolism applications in disease modeling, phenotype assays, target identification and validation, mechanism of action, and lead discovery.
Gain insight through a systems-level view of host-pathogen response through functional, kinetic metabolic measurements.
Learn how cellular bioenergetics serve as a powerful indicator of stem cell fate change, providing validation of pluripotency and lineage commitment.
Discover bioenergetic applications in neurodegenerative disease, neuroinflammatory disease, neurotoxicity, and disease modeling.
Gain an accurate picture in lead optimization with real-time metabolic measurements as a sensitive measure of mitochondrial and safety toxicology.
Learn how cellular metabolism can advance your cardiovascular research—in disease modeling, preclinical safety and toxicity, and early drug discovery.
Explore applications of cellular metabolism for environmental and mitochondrial toxicity, and environmental stressors in model organisms.
Reveal deeper insights into cell biology and critical drivers of disease with combined live cellular metabolism and metabolic approaches.
Learn how the Seahorse XF Imaging & Normalization System delivers a robust solution for resolving XF data.
View our Agilent Cell Analysis eSeminar series to explore our wide selection of eSeminars, available to view on demand. In this webinar series, we invite researchers to share their research using Seahorse XF technology. Discover how other researchers are using real-time, metabolic measurements to advance their research.
Check out the Agilent Cell Analysis Publication Database for a curated collection of over 6,800 Agilent Seahorse XF peer-reviewed publications. Explore the Publication Database search filters to query Seahorse XF publications by research area, cell line, cell type, author, assay, or analyzer to identify publications relevant to your research.
Seahorse Analyzers provide routine, accurate, and reliable measurements that may be leveraged to support multiple specific aims within a grant. Streamline and improve your research proposals to include Seahorse XF technology with the Agilent Seahorse XF Grant Expeditor Package highlighting key features and justification of these critical measurements. The free XF Analyzer Grant Expeditor Package can simplify your grant application process.
