How Agilent Seahorse XF Analyzers Work

Seahorse consumables are developed specifically for use with the Seahorse XF Analyzer. They provide sensitive, kinetic measurements to simultaneously analyze the two main metabolic energy pathways: mitochondrial respiration and glycolysis.

Seahorse XF FluxPaks include sensor cartridges, tissue culture plates, calibrant, and utility plates for performing calibration. The sensor cartridges contain solid state sensor probes for detecting oxygen and proton levels in cell culture media. They also have integrated injection ports for introduction of stimuli, inhibitors, and substrates during the assay which enables monitoring transient cellular responses in real time. The XF tissue culture plates are provided in 8-well, 24-well and 96-well formats. Specialized cell culture plates have been designed for spheroids and islets. Every lot of XF Fluxpaks and culture plates is 100% quality controlled for performance and lot-to-lot consistency.

Seahorse XF kits, reagents and media are designed for performing a wide range of assays with XF analyzers and XF Fluxpaks, to study a range of cellular function, including metabolic phenotyping, ATP production, mitochondrial function, fatty acid oxidation, and glycolysis in real time. They are standardized, optimized open-and-go products that allow to achieve consistent results, reduce assay complexity and improve research productivity. Each lot of products are rigorously tested and validated with cells to deliver high performance and low lot-to-lot variation. Together with Agilent's excellent technical support, these kits, reagents and media will greatly enhance your overall experience of performing XF assays.