QuikChange Primer Design

The QuikChange® Primer Design Program supports mutagenic primer design for your QuikChange mutagenesis experiments. Using primer design guidelines described in QuikChange manuals, this program calculates/designs the appropriate primer sequences with the optimal melting temperature. Read Help for more information about the program.
1. It is recommended that you clear this form prior to loading each new sequence:
2. Select QuikChange® mutagenesis kit that you are using:
3. Find your DNA sequence by pressing
Load a file from your hard-drive:
Or, paste plain text or FASTA-formatted DNA sequence in the box below:
4. Load it.
- or - 
or specify a DNA region to translate from to
Finally, obtain your designed primer sequences.
Clear this form to load a new sequence: