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Agilent Seahorse XF Instruments Overview and Selection Guide

Agilent Seahorse XF Instruments
Overview
Selection Guide

Overview

All Seahorse XF instruments analyze mitochondrial respiration and glycolysis in live cells, generating data in just minutes. Seahorse XF technology uses solid-state sensors to simultaneously measure both oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) in every well. All platforms support injection of up to 4 unique compounds per well.

Agilent Seahorse XF HS Mini Analyzer
XFp

The Seahorse XF HS Mini analyzer is equipped with high-sensitivity capabilities – combined with an easy-to-use interface. This makes this eight-well analyzer accessible for every lab while delivering robust XF analysis on limited cell numbers.
Agilent Seahorse XFe96 Analyzer
XFe96

The Seahorse XFe96 Analyzer provides the highest capacity for XF assays at the lowest per-sample cost. The Seahorse XFe96 enables a large number of experimental groups per assay, making it ideal for labs seeking to maximize design flexibility and sample throughput. The Seahorse XFe96 Analyzer can analyze individual spheroids with the Seahorse XFe96 Spheroid Microplate. Validated for assays performed in a hypoxia chamber.
Agilent Seahorse XFe24 Analyzer
XFe24

The Seahorse XFe24 is validated by hundreds of peer-reviewed publications, and utilizes a larger well format which is suitable for islets and other specialty sample types. Validated for assays performed in a hypoxia chamber.

Selection Guide


Agilent Seahorse XF HS Mini
Analyzer
Agilent Seahorse XFe96
Analyzer
Agilent Seahorse XFe24
Analyzer
Plate Format Miniplate Microplate Microplate
Number of Assay Wells 8 96 24
Microchamber volume 2 µL 2 µL 7 µL
Assay Working Volume 150-275 µL 150-275 µL 500-1000 µL
Recommended Injection Volume 25 µL 25 µL 75 µL
Controller Integrated full-color touch-screen interface to design basic protocols and run imported templates. Combination computer and touchscreen display with full assay design, control, and analysis capability. Combination computer and touchscreen display with full assay design, control, and analysis capability.
Software Create and run standard assays on controller and perform data analysis with Seahorse Analytics. Design and analyze assay templates on Controller or Wave desktop. Design and analyze assay templates on Controller or Wave desktop.
Best For
  • Pairwise comparisons
  • Phenotyping single samples
  • Precious biomaterial
  • Ex-vivo and primary cells
  • Phenotypic Screening
  • Testing many conditions at one time
  • Dose-response studies
  • Spheroids
  • Islets
  • Larger samples
Key Advantages
  • Easy to set up and run via streamlined interface
  • Standardized protocols for XF Assays
  • Validated for assay temperatures 16-40°C (8–20°C lower ambient temp required)
  • Maximum experimental flexibility
  • Highest throughput
  • Validated for hypoxia
  • Validated for assay temperatures 16–42°C (12–20°C lower ambient temperature required)
  • Validated for hypoxia
  • BValidated for assay temperatures 16–42°C (12–20°C lower ambient temperature required)
  • Balances throughput and budget considerations

The Agilent Seahorse XFe and XF HS Mini analyzers feature:


See more information on How Agilent Seahorse XF Analyzers work.

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For Research Use Only. Not for use in diagnostic procedures.