TekTalk

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TekTalk Newsletter Archive

Review past issues of TekTalk, BioTek's complimentary life science instrumentation e-newsletter. If you are not currently subscribed, you may sign up now to begin receiving issues as they are released. Our current distribution is 6 issues/year.

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Histology and Slide Scanning
March 2022

Histology and Slide Scanning

In this TekTalk we’ve compiled a variety of resources that describe the unique combination of hardware and software features available with the Agilent BioTek imaging systems for conducting histology and slide scanning applications, as well specific tips and guidance for optimizing your workflows.

Featured Applications: Hematoxylin and Eosin Stained Tissue and Detection and Automated Imaging of Regions of Interest (ROIs) when Performing Whole Slide Imaging (WSI)

Featured Product: BioTek Cytation 7 Cell Imaging Multimode Reader

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Cancer Research
January 2022

Cancer Research

In this TekTalk we focus on how proliferation and colony formation assays can be utilized, providing a brief description of the assay principles, and guidance on selecting the right assay format.

Featured Applications: Automated Kinetic Imaging Assay of Cell Proliferation in 384-Well Format and Automated Colony Formation Assay

Featured Product: BioSpa Live Cell Analysis System

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Lamp Assays
September 2021

Lamp Assays

In this TekTalk we demonstrate the use of the Agilent BioTek Synergy Neo2 hybrid multimode reader to conduct optimized LAMP assays kinetically.

Featured Applications: Characterization of LAMP Assays Using a Multimode Microplate Reader and Improved LAMP Assay Performance with Increased Temperature

Featured Product: Synergy Neo2 Hybrid Multimode Reader.

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Developmental Biology: Zebrafish Applications
July 2021

Developmental Biology: Zebrafish Applications

This TekTalk features resources for fluorescence-based zebrafish applications, including both image-based and reader-based investigations.

Featured Applications: Quantitative Microscopy of Angiogenesis in Zebrafish Embryos & Whole-Organism Imaging of Spinal Motor Neurons and Musculature with the Cytation C10 Confocal Imaging Reader

Featured Product: Lionheart FX Automated Microscope & Cytation C10 Confocal Imaging Reader

Customer Spotlight: Zebrafish and Lionheart: A Perfect Match

Webinars: Multimodal zebrafish workflows on a single automated confocal microscope plate reader & Zebrafish Imaging with Quantitative Analysis

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Cell Counting
May 2021

Cell Counting

This TekTalk features resources for conducting and optimizing image-based cell counting applications.

Featured Applications: Automated Kinetic Imaging Assay of Cell Proliferation in 384-Well Format & Confocal Imaging and Analysis of Spheroids for Determination of Dose Response during Drug Treatment

Featured Product: Cytation C10 Confocal Imaging Reader

Webinar On Demand: Tools and Techniques for Optimizing Cell Proliferation Studies

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Confocal Fluorescence Imaging
March 2021

Confocal Fluorescence Imaging

This TekTalk features the unique capabilities of the Cytation C10 Confocal Imaging Reader and provides guidance on confocal imaging techniques.

Featured Applications: Optical Clearing for Improved Confocal Imaging of Thick Specimens & Visualizing the Mouse Retinal Vasculature using the Cytation C10 Confocal Imaging Reader

Featured Product: Cytation C10 Confocal Imaging Reader

Webinar On Demand: Cytation C10: An Affordable Confocal Imaging Reader for your Research Laboratory

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Virology
January 2021

Virology

Agilent BioTek automated imaging systems and liquid handlers provide optimized solutions for a broad range of virology applications.

Featured Applications: Automated Viral Plaque Assay Workflow Using the Cytation Cell Imaging Multi-Mode Reader & Monitoring Viral Infection of Mammalian Cells using Digital Fluorescence Microscopy

Featured Products: Cytation 7 Cell Imaging Multi-Mode Reader & MultiFlo FX Multi-Mode Dispenser

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ELISA
November 2020

ELISA

In this TekTalk we outline automated workflows for conducting ELISA assays using Agilent BioTek plate readers and liquid handlers.

Featured Applications: Detection of HTLV I and II Antibodies using Absorbance-based ELISA & Automated ELISA Liquid Handling with the EL406 Combination Washer Dispenser

Featured Products: Epoch 2 Microplate Spectrophotometer & EL406 Washer Dispenser

Webinar On Demand: ELISA: Best Practices and Troubleshooting

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Immune Cell-Mediated Cytotoxicity
September 2020

Immune Cell-Mediated Cytotoxicity

Optimized imaging-based workflows for both 2D and 3D cell mediated cytotoxicity assays using Agilent BioTek automated live cell analysis systems.

Featured Applications: An Image-Based Method to Detect and Quantify T Cell-Mediated Cytotoxicity of 2D and 3D Target Cell Models & Validation of an Image-Based 3D Natural Killer Cell-Mediated Cytotoxicity Assay

Featured Product: BioSpa Live Cell Analysis System

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Microbiology
July 2020

Microbiology

Overview of the broad range of microbiology applications enabled by Agilent BioTek automated imagers, plate readers, & purpose-build LP600.

Featured Applications: Monitoring Bacterial Growth under Different Environmental Conditions & Multi-Mode Metabolic Profiling of Stationary Phase Saccharomyces Cerevisiae Exposed to Short-Term Oxidative or Nutrient Stress

Featured Products: LogPhase 600 Microbiology Reader & BenchCel Microplate Handler

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Cell Metabolism
May 2020

Cell Metabolism

Agilent BioTek plate readers and automated imaging systems provide versatile and powerful platforms for conducting cell metabolism studies.

Featured Applications: Optimization of a Multi-Mode Detection Model for Measuring Real-Time Cellular Respiration and Mitochondrial Function using Fluorescent Biosensors & XF Data Normalization by the Agilent Seahorse XF Imaging and Normalization System

Featured Products: Cytation 5 Cell Imaging Multi-Mode Reader

Containment Enclosures and Biosafety Cabinets for BioTek Instrumentation

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ELISpot
March 2020

ELISpot

Agilent BioTek imagers provide a full range of automated solutions for conducting ELISpot assays across all commonly used formats.

Featured Applications: Stimulation of Human Peripheral Blood Mononuclear Cells & Automating the Wash Steps of ELISpot Assays using BioTek’s 405 TS Microplate Washer

Featured Products: Cytation 7 Cell Imaging Multi-Mode Reader & 405 TS Washer

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Genotoxicity
January 2020

Genotoxicity

In this TekTalk we describe automated imaging and analysis methods for two common genotoxicity assay formats—the comet assay and gH2AX assay.

Featured Applications: Automated Imaging and Dual-Mask Spot Counting of γH2AX Foci to Determine DNA Damage on an Individual Cell Basis and Automated Comet Assay Imaging and Dual-Mask Analysis to Determine DNA Damage on an Individual Comet Basis

Featured Products: Cytation 7 Cell Imaging Multi-Mode Reader

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Spheroids - 3D Cell Culture
October 2019

Spheroids - 3D Cell Culture

In this TekTalk issue we demonstrate the diverse ways in which Agilent BioTek instruments enable 3D cell culture applications.

Featured Applications: Long-Term Hepatotoxicity Studies using Cultured Human iPSC-Derived Hepatocytes & Automated Media Exchange for Spheroid Cultures Using a Novel MultiFlo FX Accessory

Featured Product: Automated, Gentle Media Exchange

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Micro-Volume Analysis
August 2019

Micro-Volume Analysis

This issue of TekTalk describes the use of the Take3 Micro-Volume Plate with our Agilent BioTek plate readers for micro-volume analysis.

Featured Applications: Micro-volume Nucleic Acid Analysis using Common Assay Methods & Micro-volume Nucleic Acid Analysis using Common Assay Methods

Featured Product: Take3 Micro-Volume Plates

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Cell Migration
June 2019

Cell Migration

In this edition of TekTalk, we focus on a fully automated cell migration assay using the Agilent BioTek AutoScratch Wound Making Tool.

Featured Applications: Advanced Wound Healing Assay Workflow using Automated Scratch Wound Creation, High Contrast Brightfield and Fluorescence Kinetic Imaging & Automated Label-Free Method for Measuring Cell Migration in Real-Time with Oris Pro Assay

Featured Products: Cytation 5 with Wide Field of View Camera & Scratch Assay Starter Kit

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Live Cell Imaging
April 2019

Live Cell Imaging

In this issue of TekTalk we show how the Agilent BioTek automated imaging systems enable kinetic live-cell experiments over broad timescales.

Featured Applications: Thapsigargin-induced Cellular Stress Response and Inhibition of Gq-dependent Calcium Signaling and Monitoring pH in Long-term Proliferation Assays

Featured Product: BioSpa Live Cell Analysis System

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Nucleic Acid Quantification
February 2019

Nucleic Acid Quantification

This edition of TekTalk covers many applications of nucleic acid quantification and describes optimized workflows for Agilent BioTek instruments.

Featured Applications: Analytical Performance of Nucleic Acid Micro-Volume Quantification Using the Epoch Spectrophotometer System and Quantification of dsDNA using Qubit dsDNA Broad Range (BR) Assay Kit

Application Guide: Spectrophotometric Nucleic Acid Quantification in Microplates and Micro-Volumes

Featured Product: Epoch 2

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Cell Counting
December 2018

Cell Counting

In this edition of TekTalk we describe the use of cell counting for a range of applications, including label-free cell proliferation studies.

Partner Spotlight: Agilent Technologies

Featured Applications: Kinetic Proliferation Assay Using Label-Free Cell Counting and Live Cell Imaging of Apoptosis and Necrosis

Featured Product: Cytation 1 and 5 Imagers - Automated Normalization Through Cell Counting

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3D Cell Culture
October 2018

3D Cell Culture

This issue of TekTalk focuses on 3D cell culture methods and associated assays, providing a variety of resources and included tips and tricks.

Customer Spotlight: StemTek Therapeutics

Featured Applications: 3D Spheroid-Based Tumor Invasion Assay and 3D Cell Culture: A Review of Current Techniques

Featured Product: New! AMX Automated Media Exchange for MultiFlo FX

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