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How to run an assay

running an assay
Procedures, techniques & resources for a successful Assay
Your XF Instrument Type
This learning center is designed to introduce you to the Seahorse XF assay workflow, with a focus on procedures and techniques to ensure optimal XF assay performance and results. As you read through each section, the procedures refer to using the Agilent Seahorse XF Cell Energy Phenotype Test Real Time ATP Rate Assay to perform initial cell characterization. The techniques described apply to all Seahorse XF Assays, such as seeding adherent cells, loading injection ports, etc. Only the required consumables will vary according to your XF Analyzer model and XF Assay Kit. Select your XF Analyzer using the drop-down menu, then click a section below to display the relevant content for that step of the XF assay workflow.

Select a step to display content

+ Gather Assay Materials

+ Prepare for Your XF Assay

+ Set up your XF Assay

+ Run your XF Assay

+ Analyze XF Assay Results

+ Beyond the Basics

Need more information?
Worldwide Technical Support
USA and Canada
1-800-227-9770; select option 3 then 8
UK: 0800 096 7632
Germany: 0800 180 66 78
Netherlands: 0800 022 7243
Other EU: +45 3136 9878
Shop the online store to purchase cartridges, plates, kits, media, and reagents to your your XF assay. Browse the complete list of all for your .
Get in touch with an Agilent Seahorse XF representative to schedule a demonstration and see how Agilent Seahorse XF Analyzers can solve your research needs.
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For Research Use Only. Not for use in diagnostic procedures.