The Agilent Advantage – Measure Immune Cell Activation in Minutes
Cellular energy metabolism is now understood to influence many immune cell
pathways as both an indicator and controller of immune cell function and
lineage commitment. This concept is clearly illustrated in the activation
of naive T cells to stimulate proliferation and macrophages to stimulate
pro-inflammatory signaling.
Expand these sections below to learn more about accelerating your immune cell research with XF technology.
Immune Cell Activation Application Notes & Technical Information
+ T CELL ACTIVATION Real-time analysis of in situ activated T cells
Measure and Discriminate T cell
Activation in Real-Time ![]() |
BIBLIOGRAPHY
POSTERS
WEBINAR
XF TECHNOLOGY OVERVIEW
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+ MACROPHAGE ACTIVATION Real-time analysis of in situ activated macrophages
Measure and Discriminate Macrophage Activation in Real-Time
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BIBLIOGRAPHY
WEBINAR
XF TECHNOLOGY OVERVIEW
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+ NEUTROPHIL ACTIVATION Real-time analysis of in situ activated neutrophil
Direct Detection of Neutrophil
Oxidative Burst
in Real-Time ![]()
Agilent Seahorse XF technology enables real time detection and quantification of neutrophil activation by simultaneously measuring the oxygen consumption rate (OCR), oxygen consumed when ROS are generated, and proton efflux rate (PER; a measure of glycolysis) required for ROS production. Bioenergetic response as measured by XF technology is required for neutrophils to meet their rapidly changing cellular energy and metabolite demands.
Simultaneous measurements of OCR and PER are also not possible with other methods. Within the application note, the utility of the XF neutrophil activation assay can be expanded to enable studies of innate immune responses to modulators, drug treatments and micro environment cues.
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BIBLIOGRAPHY
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Activate
- Real-time discrimination of immune cell activation responses.
Modulate
- Introduce up to 4 modulators per well with programmable drug injection ports to elucidate immune cell response to activation kinetics.
Investigate
- Explore the metabolism driving immune cell response through assays providing metabolic phenotype, bioenergetic pathway, and fuel/substrate preference.

Adapted from Hongbo Chi, Nat Rev Immunol.;
12(5): 325-338, 2012
12(5): 325-338, 2012
The Cell Lineage and Immunometabolism Link
Advances in the field of immunology show cellular metabolism as a driver and regulator of immune cell function and differentiation.
T cell and macrophage activation are correlated with metabolic shifts toward glycolysis, activating energy promoting pathways required for high rates of proliferation or signaling.

Modulate immune cell activation
with the use of programmable drug injection ports
with the use of programmable drug injection ports
Agilent Seahorse XF sensor cartridges incorporate integrated injection ports that sequentially deliver up to 3 compounds, in addition to immunogenic activators, allowing dose response, agonist or antagonist response, or pathway perturbation analysis of each sample.
Dive deeper with standardized cell metabolism assays and powerful software
In addition to T cell and macrophage activation, Seahorse metabolic assay kits are available for measuring mitochondrial respiration, glycolysis, and fatty acid oxidation in cells. Seahorse XF kits and reagents are pre-calibrated, pre-tested, and are coupled with powerful report generator software, providing functional metabolic data from live immune cells.
Metabolic Phenotype
Rapidly determines the energy phenotype/fingerprint of cells and reveals their metabolic potential

Mitochondrial Profiling
Provides a complete
mitochondrial profile of your immune cells using the
mitochondrial stress test kit

Glycolysis Profiling
Provides a complete
glycolytic profile of your immune cells using the
glycolytic stress test kit

Don’t wait to accelerate your research!
Learn more about XF Technology.
For Research Use Only. Not for use in diagnostic procedures.