Seahorse XF Cell Mito Stress Test kit
Seahorse XF Mito Stress Test Kits
A new version of this kit, the Seahorse XF 3D Mito Stress Test kit, provides higher amounts of reagents in order to perform functional assays with 3D models such as tissues, organoids and small organisms.
For Research Use Only. Not for use in diagnostic procedures.
- XF Assay Kits, Reagents & Cell Assay Media
Product Details
- The assay measures basal respiration, ATP-linked respiration, H+ (proton) leak, maximal respiration, spare respiratory capacity, and non-mitochondrial respiration using modulators of cellular respiration
- Kits contain the appropriate reagent amounts to support the assay with cells or with 3D models
- Compounds are stored at room temperature and reconstituted directly in assay medium, eliminating the need for DMSO or other solvents
- Each lot is rigorously tested and verified with functional assays to deliver consistent and robust results, eliminating the costs associated with failed experiments
Automatic measurement of energy metabolism in real time
Agilent Seahorse XF Analyzers measure oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a multi-well plate, interrogating key cellular functions such as mitochondrial respiration and glycolysis. Seahorse XF Analyzers perform compound addition and mixing, label-free analytical detection, and automatic measurement of OCR and ECAR in real time.
Learn More- Application Notes
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A Superior System for Real-Time Metabolic Analysis with Brain Tissue and Other 3D Models
An optimized Agilent XF 3D tissue workflow that allows for detection and quantitative assessment of energy metabolism in tissue materials is presented.
- Application Notes
- English
- 29 Apr 2025
- 3.07 MB
Gaining Insights into Disease Biology for Target Identification and Validation
This Application Note shows how Seahorse XF technology can be used to determine specific metabolic states associated with cellular dysfunction and disease
- Application Notes
- English
- 12 Nov 2019
- 572.72 KB
Direct Measurements of Cellular Metabolism for Identification of Mitochondrial Drug Targets
An overview of how the Agilent Seahorse XF Cell Mito Stress Test and XF PMP workflows can be applied to mitochondrial and metabolic target identification studies.
- Application Notes
- English
- 10 Jun 2019
- 389.41 KB
Identifying Metabolic Switches Using an Agilent Seahorse XFe Analyzer in Hypoxia
Identifying Metabolic Phenotype Switches in Cancer Cells Using the Agilent Seahorse XF/XFe Analyzer in an Hypoxic Environment
- Application Notes
- English
- 03 Jan 2017
- 744.28 KB
Measure Mitochondrial Respiration in Skeletal Muscle Fibers with a Seahorse Analyzer
Measuring Mitochondrial Respiration in Intact Skeletal Muscle Fibers with an Agilent Seahorse XF24/XFe24 Analyzer
- Application Notes
- English
- 22 Dec 2016
- 1.07 MB
Assessing therapies for traumatic brain or spinal cord injuries
XF technology provides insight into treatment interventions for mitochondrial dysfunction
- Application Notes
- English
- 22 Dec 2016
- 679.93 KB
Assessing the impact of autophagy on cellular metabolism
Real-time, functional metabolic assays connect energy pathways and autophagy
- Application Notes
- English
- 22 Dec 2016
- 1.06 MB
- Flyers
- Posters
- Selection Guides
- Technical Overviews
Agilent Cell Analysis Publication Database
Search scientific publications by research area, cell type and more.
Seahorse XF Assay Learning Center
Up-to-date information on protocols and procedures for preparing, running, and analyzing an XF assay.
Customer Reviews for Seahorse XF Cell Mito Stress Test Kit
Explore customer reviews of this product or write your own review
Agilent Cell Analysis Webinars
Featured webinars help you stay informed about timely cell analysis research topics.
The Agilent Seahorse XF Pro platform
Transforming Bioenergetics - Again. Featuring new pharma-oriented workflow solutions with improved precision, along with advanced experimental design and analysis tools, you can optimize your workflow from start to finish.
- 17 Jan 2022
The Seahorse XF Flex Analyzer: Powerful Metabolic Insights in 2D & 3D Models
The 24-well Agilent XF Flex Analyzer offers enhanced sensitivity, a broad detection range, and compatibility with 3D models like organoids, tissues, and co-cultures. Purpose-built consumables and powerful analysis tools help you design smarter experiments, extract deeper insights, and generate translatable data—empowering your research to reach new heights in cellular metabolism and function.
- 14 Apr 2025
- Material Safety Data Sheets
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Seahorse XF Cell Mito Stress Test Kit
Material Safety Data Sheet
- Material Safety Data Sheets
- English
- 04 Jun 2025
- 195.93 KB
Seahorse XF 3D Mito Stress Test Kit
Material Safety Data Sheet
- Material Safety Data Sheets
- English
- 05 Mar 2025
- 197.87 KB
Seahorse XFp Cell Mito Stress Test Kit, Part Number 103010-100
Material Safety Data Sheet
- Material Safety Data Sheets
- English
- 14 Dec 2023
- 152.50 KB
- User Manuals