Seahorse XF Mito Fuel Flex Test Kit
Seahorse XF Mito Fuel Flex Test Kit
Learn more about How XF Technology Works
For Research Use Only. Not for use in diagnostic procedures.
Product Details
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The assay uses specific inhibitors in different combinations to measure the dependency, capacity, and flexibility of cells to oxidize 3 major mitochondrial fuels: glucose (pyruvate), glutamine (glutamate), and Long-chain fatty acids, in basal energetic state.
- Dependency indicates the relative amount of basal mitochondrial oxidation from a single fuel that cannot be compensated through oxidation of the other two fuels.
- Capacity is the relative ability of a cell to oxidize a specific fuel in the basal state when oxidation of the other two fuels are blocked.
- Flexibility is the difference between Capacity and Dependency. It indicates the relative ability of a cell in the basal state to switch or compensate mitochondrial oxidation from one fuel to another.
- Compounds are reconstituted directly in assay medium – eliminating the need for DMSO or other solvents.
- Kits are rigorously tested and validated for every lot with cells to deliver consistent and robust results – eliminating the cost associated with failed experiments.
- Data analysis tool, the Seahorse XF Mito Fuel Flex Test Report Generator, is provided to simplify data processing and transform raw data into organized, shareable summary reports.
- Download Seahorse XF Mito Fuel Flex Test Report Generator
- Learn more about Mitochondrial Fuel Flexibility
Cell Reference Database - search scientific publications that reference/cite Agilent Cell Analysis products and technology by cell type, cell line, research area, assay, or author.
Publications Database - publications, in which Agilent Cell Analysis data is included, linked to PubMed.gov.
Cell Analysis Product Selection Guide - identify the best solution for your research needs.
XF Instrument Selection Guide - general specifications and features of the different Seahorse Analyzer models.
Learning Center - up-to-date information on protocols and procedures for preparing, running, and analyzing an XF assay.
Cell Analysis Travel Awards - travel awards for researchers who present a poster or presentation citing data generated using Agilent Cell Analysis technology.
XF Analyzer Grant Expeditor Package - simplify your grant application process with the Agilent Seahorse XF Analyzer Grant Expeditor Package.
XF Core Facilities - facilities that provide access to Agilent Seahorse XF technology, including advice in experimental design, data analysis, basic training, and bioinformatics on a cost recovery basis.
Lab Instrument Service Agreements – instrument service plans that maintain instrument performance and keep laboratory operations running smoothly and consistently.