Pick your Power - Cell Analysis Product Selection Guide
All Agilent cell metabolism assays measure cell metabolism by looking at oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) – it’s the technology that differs. So it’s a matter of understanding which assay best suits your biological questions, workflow, and throughput goals.
Agilent Seahorse XFp and XFe96 analyzers use solid-state sensors to simultaneously measure OCR and ECAR in every well, via the creation of a transient microchamber.
MitoXpress and pH-Xtra assays use soluble, chemically inert, cell impermeable, fluorophores to measure OCR and ECAR in real time, using plate-reader based assays.
Use this handy guide of the workflow differentiators to best understand which assay suits your needs.
Agilent Seahorse XFp and XFe96 analyzers use solid-state sensors to simultaneously measure OCR and ECAR in every well, via the creation of a transient microchamber.
MitoXpress and pH-Xtra assays use soluble, chemically inert, cell impermeable, fluorophores to measure OCR and ECAR in real time, using plate-reader based assays.
Use this handy guide of the workflow differentiators to best understand which assay suits your needs.
| MitoXpress and pH-Xtra assays | Seahorse XF assays | |
| Detection System |
TR-F enabled microplate readers. See MitoXpress Xtra, MitoXpress Intra and pH-Xtra assay User Manuals for details |
Agilent Seahorse XF, XFe or XFp Analyzers |
| Assay Format |
Any microplates suitable for TR-F measurements Suitable for 96 and 384 well formats |
XF FluxPaks Available in 8, 24 and 96-well formats |
| Sensor | Soluble, single analyte/pathway | Solid state, dual analyte/pathway |
| Assay Units | fluorescence units - relative rates [qualitative] | pmol/min - absolute rates [quantitative] |
| Assay Design Options |
Pretreatment Manual or plate reader injection possible for pH-Xtra |
Automated, sequential injection of up to four compounds per well during an experiment |
| Assay Workflow | Same day prep | Prep day prior to assay |
| Cell Seeding | Seed as normal for 96 or 384 well microplates. Typically requires 3-6 x 104 cells per 96-well | Typically requires 1-2 x 104 cells per 96-well |
| Cell Prep |
Monolayer or suspension No adherence required |
Monolayer or anchored 3D Suspension cells and microorganisms require adherence via spinning and/or plate coatings; 3D requires special plates |
| Cell Types | Moderate-to-active respiring cells and microorganisms |
Most cell types and microorganisms See Cell Reference database for details |
For Research Use Only. Not for use in diagnostic procedures.