The Seahorse XF Mito Fuel Flex Test works with any Seahorse XF Analyzer to determine the rate of oxidation of a given fuel by measuring mitochondrial respiration [the oxygen consumption rate, OCR] of cells in the presence or absence of fuel pathway inhibitors. Initial inhibition of one pathway measures how dependent the cells are on that particular fuel source to meet energy demand. Using a combination of inhibitors measures cells' capacity and flexibility in meeting energy demand. The Seahorse XFp and XF Mito Fuel Flex Test Kits contain all 3 pathway inhibitors (UK5099, BPTES and Etomoxir) required to determine the dependency, capacity, and flexibility of cells for glucose, glutamine and fatty acids, respectively. The Report Generator automatically converts the oxygen consumption rate (OCR) values acquired during the assay into a percent Dependency and Flexibility for each fuel and condition tested relative to all 3 major fuel pathways.
See more information on the Agilent Seahorse XF Mito Fuel Flex Test Kit