From Bench to Process Scale, QA/QC to Manufacturing
Over the last several decades, there has been continued interest and focus on new therapeutic modalities for the regulation of protein expression at the RNA, and even DNA level. The purification of oligonucleotides (ONs) can be particularly challenging with the high similarity of API and impurities.
Agilent recognizes the unique challenges for each oligonucleotide API, offering a range of chemistries, pore size, and dimensions to meet your needs.
Agilent offers a range of particle and pore sizes with our PLRP-S polymeric media. From bench to process, PLRP-S scales with your needs.
Purification of Single-Stranded RNA Oligonucleotides Using High-Performance Liquid Chromatography
Dynamic Binding Capacity of Oligonucleotides on PLRP-S Columns and Stationary Phases
Agilent nucleic acid purification portfolio
This application note describes common method parameters that can be optimized when purifying oligonucleotides ranging from 25 to 100 bases in length.
High Resolution Separations of Oligonucleotides using PL-SAX Strong Anion-Exchange HPLC Columns
Agilent PL-SAX for Oligonucleotide Purification. Bench to Process.
Synthetic oligonucleotides have evolved into a diverse set of molecules that vary greatly in size, structure, and modifications. With this diversity, comes many synthesis related impurities with high homology to the parent molecule.
This series of free training modules discuss the theory of oligonucleotide purification, guiding through chemistry selection, method optimization, scale-up, and API characterization in quick video modules.
Module 1 discusses the series of variables that should be considered when optimizing a purification method including yield, resolution & throughput.
Synthetic oligonucleotides can range in size from a few bases to 1000's of bases in length. The oligonucleotides can have many impurities associated with this sythesis including truncation, incomplete thiolation, base loss, and adducts. We explore the many impurities that will require purification for a pure API.
With the range of oligonucleotide sizes used as therapeutics, selection of the optimal pore size to give max binding capacity while having large enough pores for effective mass transfer is critical. This module explores performing dynamic binding capacity experiments to determine optimal pore size for your oligonucleotide purification and future scale-up.
Anion exchange (AEX) is often selected as a preferred purification method due to its low cost buffer systems that can be readily available for scale-up to manufacturing. This module discusses the optimization of AEX for improved separation by adjusting common variables such as temperature, organic, and pH.
Ion-pair reversed phase separation is often used for oligonucleotide characterization and purification. Providing high resolution separation, ion-pair reversed phase is often utilized to separate challenging, co-eluting impurities. This module discusses method optimization including temperature, pore size, ion pair selection, and future scale-up.
During oligonucleotide sythesis, purification and final formulation, monitoring of the API for final purity is critical throughput processing. This module explores the use of LC/MS for oligonucleotide characterization, including selecting an optimal ion pair and hexafluoroisopropanol (HFIP) concentration.