To successfully separate trityl-off, deprotected oligonucleotides, you need columns that have high resolving power and are robust enough to withstand challenging conditions.
If your columns dont provide enough resolution, you cant be sure your results are accurate. If your columns are not robust, you will have to replace them frequently, which will disrupt your workflow and increase your costs.
Agilent AdvanceBio oligonucleotide columns feature high efficiency, 2.7 μm superficially porous Poroshell particles. Using technology exclusive to Agilent, we chemically modify the particles to make them exceedingly resistant to high pH mobile phases. We also bond them with an end-capped C18 phase that delivers excellent selectivity for oligonucleotides. Whats more, we test every batch of AdvanceBio oligonucleotide media with a resolution standard to ensure consistent performance.
The family of AdvanceBio products are designed to deliver consistent, exceptional performance for the complete characterization of proteins, antibodies, conjugates, new biological entities and biopharmaceuticals.