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Next Generation Solutions for Immune and CAR T Cell Therapy

Tune the Drivers of
Immune Cell Potency,
Fate, and Persistence

The next generation of immune cell therapies requires a new generation of analysis tools

Develop a safe, potent, and persistent immune cell product by understanding immune cell function. Whether it be activation, proliferation, cell fate, cytotoxic killing, or immunomodulation, Agilent is dedicated to supporting your next-generation therapies with key technologies.

Breakthroughs in immunotherapy Breakthroughs in immunotherapy e-book

Download the e-book for the latest recommendations from leaders in immunotherapy research and their insights into exciting new developments.

Measuring phenotype, potency, and persistence at all phases of development
Discovery Translation Production

Develop new immune cell therapies:

  • Agilent SureGuide portfolio Perform stable, on target CRISPR editing of immune cells to engineer highly specific cell therapies.
  • Agilent xCELLigence RTCA and
    Seahorse XF technologies
    Assess and validate function, fate and fitness of immune cells on real time live-cell platforms that deliver relevant functional data.
  • NovoCyte Quanteon flow cytometer Establish actionable phenotypes at the benchtop for immune cell subsets including lineage and functional poise by multiparametric flow.

Understand the effects of immune cell therapies on a constantly evolving tumor microenvironment:

  • Test gene edits and other interventional strategies for phenotype, potency, fitness, on/off target effects, and cell fate programming.
  • Correlate relevant kinetic data to inhibitory effects such as exhaustion, anergy, and other escape phenotypes due to interactions with a constantly evolving tumor and its microenvironment.
  • Sensitively measure live cell function and simultaneously scan the temporal components of an ever-evolving immune response in terms of efficacy, persistence, and safety.

Define key quality control attributes for cell therapy manufacturing:

  • Seamlessly transition to SureGuide RNAs manufactured under GMP with the same expected quality and performance.
  • Quantify the efficacy of cell therapies to better define key quality and process performance criteria with real-time potency assays.
  • Generate reliable analytics for the quality control and release of compliant products.
Discover the Agilent immune cell therapy tool bench
Agilent xCELLigence RTCA xCELLigence RTCA
Real-time cell analysis and imaging
Agilent Seahorse XF Seahorse XF technologies
Kinetic analysis of immune cell fate
NovoCyte Quanteon NovoCyte Quanteon
Flow cytometry for immuno-oncology
on-target gene editing SureGuide CRISPR sgRNA
Stable, on-target gene editing
xCELLigence RTCA biosensor technology

Measure immune cell-mediated killing in real time xCELLigence RTCA biosensor technology provides a label-free, robust, and simple workflow, enabling measurement of diverse effector cells and molecules for cytotoxicity. It is a platform for more sensitive in vitro potency assessment that helps mimic activities in vivo.

Live cell imaging

Live cell imaging now available With the new xCELLigence eSight, live cell imaging and real-time biosensor measurements can be performed on the same cell populations, which provides important information on cell behavior.

Cell Index plot

More accurate and reproducible cytolytic data In the figure above, cytolysis of target PC3 (prostate) cancer cells by NK92 effector cells are continuously measured on the xCELLigence system for 48 hours, starting at effector-to-target ratios as low as 0.625:1.

xCELLigence Real-Time Cell Analysis (RTCA)

Quantify dynamic cell behavior that can be missed by labor-intensive endpoint methods to gain a more comprehensive measure of cancer cell killing, including potential escape phenotypes.

The next generation of CAR-T cells
Mark C. Johnson

Mark C. Johnson, PhD Team Leader
Cell Therapy Discovery

Cynthia C. Bamdad

Cynthia C. Bamdad, PhD Chief Executive Officer

Quantifying immune cell–mediated killing
Immune cell

Kristin Anderson, PhD
Vita Golubovskaya, PhD
Cynthia C. Bamdad, PhD
Dan MacLeod, PhD
Michael Overstreet, PhD

Explore functional potency assays for cancer immunotherapy research
Cancer research

New quantitative methods to monitor immune cell behavior in vitro.

Choice of T cell signaling domains impacts metabolic programming differentially

Both glycolysis and mitochondrial oxidative phosphorylation shape T cell activation and effector function. Glycolysis drives a robust, short-lived effector phenotype. In contrast, OXPHOS and fatty acid metabolism tunes persistence and sustained T cell memory formation.

Injection of anti-CD3 and anti-CD28 beads

Open an early window to detect T cell activation responses within minutes of stimulation by measuring glycolytic extracellular acidification rate (ECAR) in real time. Learn more

Agilent Seahorse XF analysis

Discover and tune immune cell programming to mount a sustained and robust antitumor response. Real-time metabolic analysis enables you to measures and tune the drivers of immune cell fate and function. Quantify activation kinetics and determine the optimal metabolic phenotype and fitness to shape product potency.

Exploring the metabolism of CAR-T cells
Roddy O'Connor

Roddy O'Connor, PhD Center for Cellular Immunotherapies
University of Pennsylvania

T cell metabolism in activation and exhaustion
Greg M. Delgoffe

Greg M. Delgoffe, PhD Assistant Professor
Tumor Microenvironment Center
University of Pittsburgh

The bioenergetics of immune cell persistence in the tumor microenvironment
David Ferrick

David Ferrick, PhD Senior Strategy Director
Cell Analysis Division
Agilent Technologies, Inc.

NovoCyte and Quanteon flow cytometers

High-performance benchtop flow cytometers designed for all levels of users. For quick and accurate immunophenotyping with up to 25 channels of fluorescence using the most sensitive silicon photomultiplier detector technology on the market. Experience the new standard in flow cytometry.

Cell proliferation diagram

Monitor T-cell expansion, compound toxicity, drug effects on tumor cell growth, immune cell behavior under different conditions, and much more.

NovoCyte software has a built-in module for cell proliferation modeling, which is shown in the diagram above. It identifies each generation of cell division, and then calculates the proliferation index.

Above, we show a 18-color pan-leukocyte immunophenotyping panel on PBMCs using the NovoCyte Quanteon, adapted from OMIP-024 (Cytometry Part A, 85A:995-998, 2014). This panel was designed to monitor different immune subsets with activation and differentiation markers.
× Above, we show a 18-color pan-leukocyte immunophenotyping panel on PBMCs using the NovoCyte Quanteon, adapted from OMIP-024 (Cytometry Part A, 85A:995-998, 2014). This panel was designed to monitor different immune subsets with activation and differentiation markers.

NovoCyte Quanteon is the most advanced benchtop flow cytometer on the market today. With four lasers and up to 25 independent photomultipliers it is ideal for conducting immuno-oncology research to support the most demanding panels. Add the NovoSampler Q for walk-away automation.

The above shows an 18-color pan-leukocyte immunophenotyping panel on PBMCs using the NovoCyte Quanteon. This panel was designed to monitor different immune subsets with activation and differentiation markers.

Simplifying cell proliferation studies with flow cytometry

Make your analysis easier with an integrated software solution

Characterizing potential biomarkers of colorectal cancer using xCELLigence & NovoCyte
Steve Offer

Steve Offer, PhD Assistance Professor of Pharmacology
Mayo Clinic School of Medicine

Application Note
Comprehensive 18-color pan-leukocyte flow cytometry analysis for immune surveillance
Plots of antibody titrations

Antibody titration for optimization of antibody concentration

18-color Pan-Leukocyte flow cytometry panel

In-depth analysis of T and NK lymphocyte activation and differentiation of PBMCs

Agilent SureGuide chemically synthesized sgRNAs

Agilent chemically synthesized guide RNAs facilitate the full potential of CRISPR for cellular engineering and immunotherapy.

These long, high quality, chemically synthesized single guide RNAs are ideal for CRISPR-based cell engineering. We offer customization options, such as modifications to increase stability and specificity, for consistent results and robust, novel therapeutics development. Small-scale research grade manufacturing and large-scale GMP manufacturing capabilities result in an end-to-end solution for your cell engineering needs–from research through clinical trials, providing quality and reliability you can depend on.


Proprietary chemistries enable the synthesis of long, high purity sgRNAs. This process facilitates the scalable manufacturing of highly pure sgRNAs, affords unique options for sgRNA design, and precise installation of molecular functionality to augment CRISPR-Cas performance.

HBB gene

Customization options include the ability to add modifications to increase stability and specificity for more consistent results. For example, adding a single modification at either position 5 or 11 of the hemoglobin subunit beta (HBB) guide RNA sequence dramatically reduced the level of off-target activity while maintaining a high level of activity at the on-target site.

Improving CRISPR performance through guide RNA enhancements
Mark DeWitt

Mark DeWitt, PhD Project Manager, Corn Group
UC Berkeley

Dan Ryan

Dan Ryan, PhD Advanced Research Scientist
Agilent Research Laboratories

Improving CRISPR-Cas specificity with chemical modifications in single-guide RNAs

Daniel E Ryan, PhD, et al. Nucleic Acids Research
Volume 46(2), 25 Jan 2018, Pages 792-803

Novel modifications and high-purity guides enhance CRISPR performance
Laurakay Bruhn

Laurakay Bruhn, PhD Biological Chemistry
Agilent Research Laboratories

Genetic Engineering & Biotechnology News
Issue 577153, Page 22

Contact us today

Want to learn more about the Agilent immune cell therapy tool bench? Whether you would like to speak to an expert, request a demo, or simply request more information, we would be happy to connect with you.

For Research Use Only. Not for use in diagnostic procedures.