SureGuide Chemically Synthesized sgRNA

RUO

Agilent’s SureGuide Chemically Synthesized sgRNAs are the industry standard for quality nucleic acids. Using a patented, proprietary chemistry¹ makes the synthesis of long RNA oligonucleotides as robust and efficient as DNA synthesis. This chemistry is used to synthesize and deliver ultra-high-quality CRISPR guides that have few contaminants. Agilent developed several unique chemical modifications that improve the efficiency, stability and specificity of guide RNAs in a variety of cell types.^{2, 3}

United States Patent:
US 10,337,001 - GUIDE RNA WITH CHEMICAL MODIFICATIONS, Ryan et al.
US20170355985 (pending) - High Specificity Genome Editing Using Chemically Modified Guide RNAs, Dellinger, et al.

Agilent’s CRISPR products licenses: Limited License Caribou Biosciences, Inc. & Limited License Broad Institute

For Research Use Only. Not for use in diagnostic procedures.

Chemically Synthesized sgRNA

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Features

Custom sequences up to 140 nt, longer lengths available upon request
Quantities from 100 µg to 100 mg (3-3000 nmol) to scale your experiment
Research and Development grade to GMP material
Chemical modifications at no extra charge (M or MS at 5' and 3' ends)
Additional modifications and longer lengths available upon request, ensuring the flexibility you need
Click here to request a quote for your sequence

Featured References

Publications

Dellinger et al. “Streamlined Process for the Chemical Synthesis of RNA Using 2’-O-Thionocarbamate-Protected Nucleoside Phosphoramidites in the Solid Phase”, J. Am. Chem. Soc. 133, 11540–11556 (2011).
Ayal Hendel, Rasmus O Bak, Joseph T Clark, Andrew B Kennedy, Daniel E Ryan, Subhadeep Roy, Israel Steinfeld, Benjamin D Lunstad, Robert J Kaiser, Alec B Wilkens, Rosa Bacchetta, Anya Tsalenko, Douglas Dellinger, Laurakay Bruhn & Matthew H Porteus. Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells. Nature Biotechnology, volume 33 number 9 (2015); doi:10.1038/nbt.3290
Daniel E. Ryan, David Taussig, Israel Steinfeld, Smruti M. Phadnis, Benjamin D. Lunstad, Madhurima Singh, Xuan Vuong, Kenji D. Okochi, Ryan McCaffrey, Magdalena Olesiak, Subhadeep Roy, Chong Wing Yung, Bo Curry, Jeffrey R. Sampson, Laurakay Bruhn, and Douglas J. Dellinger. Improving CRISPR–Cas specificity with chemical modifications in single-guide RNAs. Nucleic Acids Research, Vol. 46, No. 2 (2018); doi: 10.1093/nar/gkx1199

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