Clinical Diagnostic Imaging Reader, BioTek Cytation 5

BioTek Cytation 5 Cell Imaging Multimode Reader

IVD

Cytation 5 combines automated microscopy and conventional microplate detection in a configurable, upgradable platform for the clinical laboratory. The microscopy module offers up to 60x magnification in fluorescence, brightfield, high contrast brightfield, color brightfield, and phase contrast to address many applications and workflows.

The multimode detection modules include filter- and monochromator-based fluorescence detection, luminescence, and UV-Vis absorbance detection. Gen5 IVD software provides features for IVD compliance requirements.

For In Vitro Diagnostic Use.

Clinical Imaging Multimode Readers

Features

Fully validated for IVD use
Gen5 IVD software provides features for IVD compliance requirements
Modular, upgradable imaging modes, plus multimode detection enables a range of cell-based assays and applications beyond a standard microplate reader
Increase productivity with a system that is ready for any assay, with a combination of hybrid plate reader and advanced microscopy mode
Fully automated microscopy tasks, from slide scanning to time lapse live cell assays, and flexible hardware to increase productivity and reduce manual labor
A combination of monochromator and filter optics delivers flexibility and performance to microplate assays. A choice of optics allows you to read microplates in a wide range of applications
BioTek Gen5 software enables walkaway automation for your image process and analysis workflows and data analysis tasks
Variable bandwidth quad monochromator optics enhance assay performance including increased sensitivity and lower limits of detection

Specifications


Automation Compatibility	BioSpa BioStack 3rd-party BenchCel
Camera	Sony CMOS, 16-bit color
Environment Control	4-Zone Incubation to 65 °C Condensation Control Peltier Cooling CO2, O2 Control
Filter Detection Modes	BRET Fluorescence Intensity Fluorescence Polarization Luminescence Time Resolved Fluorescence TR-FRET Alpha (Laser)
Imager/Reader Options	Joystick stage control Laser autofocus Reagent injectors
Imaging Methods	Montage Multi-color Single-color Time lapse Z-stacking
Imaging Modes	Widefield fluorescence Brightfield High contrast brightfield Color brightfield Phase contrast
Magnification Range	1.25x to 60x air 4x to 40x phase contrast
Microscope Type	Inverted microscope
Monochromator Detection Modes	UV-Vis Absorbance Fluorescence Intensity (variable bandwidth) Luminescence Time Resolved Fluorescence (secondary)
Shaking	Linear Orbital Double-orbital
Supported Labware	6 to 1536-well plates Slides Cell culture dishes Cell culture flasks (T25) Hemocytometers Chamber slides Take3/Take3 Trio

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Technology

Hybrid plate reader: Flexibility and performance

With its patented combination of monochromator and filter optics, Cytation 5 is an advanced plate reader that delivers both the flexibility and performance you need for any microplate assay in your lab. Monochromator: variable bandwidth, absorbance, fluorescence, luminescence Filters: fluorescence polarization, time-resolved fluorescence, Alpha laser.

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Variable bandwidth for sensitivity and specificity

The plate reader optics of Cytation uses a quad monochromator design with variable bandwidth. The bandwidth can be set anywhere between 9 and 50 nm in 1 nm increment. Large bandwidth settings (1) provide increased sensitivity and lower limits of detection. Small bandwidth settings (2) provide increased specificity when multiple signals are present, which reduces signal crosstalk and enhances assay performance.

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Peltier cooling for environmental stability

The Peltier Cooling Module promotes a rapid interior cool down after incubated processes, allowing efficient switching between multiple applications. For temperature sensitive assays run at room temperature, the Cooling Module maintains temperature, allowing < 1 °C rise regardless of internal or external fluctuation.

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Patented laser autofocus

Laser autofocus and image-based (or software-based) autofocus are two methods of autofocus widely used in microscopy.

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Time-resolved fluorescence

Time-resolved fluorescence (TRF) measurement is very similar to fluorescence intensity (FI) measurement. The only difference is the timing of the excitation / measurement process.

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Alpha detection

AlphaScreen is a bead-based assay technology used to study biomolecular interactions in a microplate format. The acronym 'Alpha' stands for amplified luminescent proximity homogeneous assay. This platform provides a nonradioactive, homogeneous assay that has low background and high signal to background ratios.

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Absorbance

Absorbance refers to the ability of a substance to absorb light of a specific wavelength. In a microplate reader designed to measure absorbance, a light source illuminates the sample using a specific wavelength, selected by an optical filter or a monochromator.

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Fluorescence polarization

Fluorescence polarization (FP) measurements are made using an optical system that includes polarizing filters in the light path. Samples in the microplate are excited using polarized light. Depending on the mobility of the fluorescent molecules found in the wells, the light emitted will either be polarized or not.

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TR-FRET

Time-resolved fluorescence energy transfer (TR-FRET) works on the principles that when suitable pairs of fluorophores are in close proximity of one another, excitation of a lanthanide donor fluorophore results in energy transfer to an acceptor fluorophore.

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How It Works

BioTek Cytation 5 Cell Imaging Multimode Reader

Product Details

Hybrid plate reader: Flexibility and performance

Variable bandwidth for sensitivity and specificity

Peltier cooling for environmental stability

Patented laser autofocus

Time-resolved fluorescence

Alpha detection

Absorbance

Fluorescence polarization

TR-FRET

Customer Reviews for BioTek Cytation 5 Cell Imaging Multimode Reader

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