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AdvanceBio 脱盐反相色谱柱
在质谱检测之前提供简单快速的反相盐离子去除,这些色谱柱可以使用传统的质谱不兼容技术(如 IEX、SEC 和亲和色谱)进行质谱检测。使用这些色谱柱可大大减少质谱检测器污染,有助于大幅缩短仪器维护和停机时间。
- Intact & Subunit Analysis
Product Details
- 允许使用传统质谱不兼容技术(如 IEX、SEC 和亲和色谱)进行质谱检测。
- 对样品在线脱盐,节省时间。
- 洗脱前浓缩样品
- 通过保持质谱仪清洁,缩短停机时间,减少维护。
- 提高数据质量,从而提高整体数据可信度。
- 适用于 1000 Å 大孔径的生物大分子
- 在 pH 2–13 的范围内稳定
- 与所有常用反相流动相兼容
- Application Notes
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Efficient Monoclonal Antibody and Antibody Drug Conjugate Desalting Prior to Mass Spectrometry using AdvanceBio Desalting-RP Cartridges
Presents the use of AdvanceBio Desalting-RP cartridges to pretreat mAb and ADC samples before MS analysis.
- Application Notes
- English
- 01 Nov 2018
- 2.11 MB
Cell Clone Selection Using the Agilent Bio-Monolith Protein A Column and LC/MS
An Agilent Bio-Monolith Protein A column determined recombinant mAb titer and was used to enrich µg amounts of antibody for further structural characterization by MS.
- Application Notes
- English
- 22 Nov 2017
- 264.06 KB
2D-LC as automated desalting tool for MSD analysis
Demonstion of the use of multiple heart-cutting two-dimensional liquid chromatography for automated desalting of glucagon from an MS incompatible USP method.
- Application Notes
- English
- 05 Sep 2017
- 2.15 MB
Agilent AdvanceBio Desalting-RP Cartridges for Online Desalting in 2D-LC/MS mAb Analysis
Use of a desalting cartridge in the second dimension to desalt and characterize monoclonal antibodies (mAbs) using a 2D-LC/MS setup.
- Application Notes
- English
- 16 Sep 2016
- 749.05 KB
Application of AdvanceBio Desalting - RP Cartridges for LC/MS Analysis of mAbs
AdvanceBio Desalting - RP cartridges used for effective desalting of LC-separated monoclonal antibodies (mAbs) before characterization by mass spectrometry
- Application Notes
- English
- 03 Aug 2016
- 889.38 KB
Characterize Fab and Fc Fragments by Cation-exchange Chromatography
Agilent Bio Mab WCX separates charged variants of papain-digest monoclonal antibody, following by MS characterization of the charged variants with online desalting.
- Application Notes
- English
- 04 Dec 2014
- 279.37 KB
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安捷伦推出全新 AdvanceBio 离心柱,用于脱盐和缓冲液置换
AdvanceBio 离心柱可满足在水溶液条件下实现高效的蛋白质或寡核苷酸样品前处理,通过合适的离子强度和 pH 保留天然结构的需求。用户可以轻松、快速地从样品基质中去除过量的盐或不需要的小分子,或者使用不同的缓冲液来置换样品基质。这些凝胶过滤型离心柱有用于单个样品的分析型和半制备型色谱柱,以及用于高通量分析的 96 孔样品板。
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