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Intact & Subunit Analysis

Wide Pore LC Columns for Intact Protein & Subunit Analysis

Agilent offers a number of wide pore reversed-phase columns. From superficially porous, silica-based AdvanceBio RP mAb to totally porous, polymer-based PLRP-S, there is a column solution for every intact or subunit protein separation.

Intact & Subunit Analysis

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Featured Tools

Bio HPLC Columns eSelector Tools

Intuitive process to help you select the right chromatography column for your biopharmaceutical applications.

Preparative HPLC Scaling Calculator

Scale between analytical and prep methods; calculate column capacity, # of injections, run time & mobile phase use.

SEC Columns FAQs

A guide to SEC columns, including tips for success and troubleshooting.

eCertificates of Product Quality and Analysis

Get a copy of your product’s certification using Part and Serial/Lot number info.

Featured Customer Testimonials

Piotr Alvarez

Senior Scientist, RIC Group

The deactivated HILIC-Z column substantially reduced the undesired analyte-surface interactions in the analysis of GLP-1 receptor agonist drug products. We observed at least a 30% increase in sensitivity and reduction in peak tailing for the analysis of acidic peptides and excipients. Peptide impurities eluting in the peak tail were hardly detectable when using conventional stainless steel hardware.

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Koen Koen

CEO, RIC Group

The novel hardware brings hidden realities into view.

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Altura HPLC Columns with Ultra Inert Technology

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Featured News

Meet the New Altura Family of HPLC Columns

Altura columns feature Agilent's innovative Ultra Inert technology. The advanced coating blocks active metal sites, ensuring an inert flow path while maintaining the strength, pressure tolerance, and consistency of a traditional stainless steel HPLC column. Altura columns unlock the true separation potential of the stationary phase. Experience superior chromatographic performance, faster equilibration, and enhanced sensitivity for your most challenging metal-sensitive analytes.

  • 01 Oct 2025

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