Cary 3500 Multicell UV-Vis Spectrophotometer
Cary 3500 Multicell UV-Vis Spectrophotometer
The Cary 3500 comes complete with Cary UV Workstation software—a next-generation analytical platform with streamlined methods for wavelength reads, scanning, concentration, kinetics, and temperature measurements. When configured with Agilent OpenLab data management software, Cary UV Workstation data is managed, protected, and stored in a local or central secure database.
- UV-Vis & UV-Vis-NIR Instruments
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Product Details
- Measure samples at four temperatures across eight cuvette positions in a single experiment and collect data from each cuvette simultaneously.
- Control the temperature of your samples accurately and quickly from -5 to 110 °C without water, noise, or messy cables using a unique and fully integrated air-cooled Peltier system.
- Control UV-Vis thermal ramp measurements at up to 40 °C/min using highly accurate, solid-state digital Cary temperature probes that control experimental temperature from inside the cuvette.
- Ensure analytical accuracy for small-volume measurements with a highly-collimated and uniform beam of less than 1.5 mm width, coupled with permanent optical alignment of the stationary cell holders.
- Reduce data processing time and calculation errors with more than 50 built-in and customized calculations that can be saved and applied automatically to any method used routinely.
- Achieve research grade photometric performance, stability, and range with a double beam spectrophotometer featuring leading optical technologies.
- Be up and running in minutes by following the intuitive video tutorials in Cary UV Workstations Help and Learning Center.
- Meet data integrity standards—provides the technical controls to securely acquire, process, report and store data in laboratories that must follow the compliance guidelines of FDA 21 CFR Part 11, EU Annex 11, GAMP5, as well as ISO/IEC 17025 and EPAs 40 CFR Part 160.
- Store and manage your Cary 3500 Multicell UV-Vis spectrophotometer data in a local secured storage on the workstation PC, or in a central storage location.
- Deploy the OpenLab software suite and get optional secure database storage for electronic records, complete administration for users and access privileges, software licensing, and security settings—e-signature workflow and enhanced audit trail review capabilities.
- Choose sustainability: The Cary 3500 Multicell UV-Vis received My Green Labs ACT (Accountability, Consistency, Transparency) label after independent audit for its environmental impact throughout the product lifecycle.
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Xenon Flash Lamps for UV-Vis & Fluorescence Spectrophotometers
For more than 35 years, Agilent has been the pioneer in xenon flash lamp technology for UV-Vis and fluorescence instrumentation. Agilent provides innovative workflow solutions using xenon lamps, including our 10-year lamp warranty, on Cary 60 UV-Vis, Cary 3500 UV-Vis, and Cary Eclipse Fluorescence spectrophotometers.
Learn MoreUV-Vis Analysis of Biomolecules
Analyzing proteins, oligos, monoclonal antibodies, and other biomolecules? UV-Vis spectroscopy is a key part of any bioanalytical toolkit, with many features and benefits that accelerate workflows involving biomolecules. Read our guide and customer case studies to learn more.
Learn MoreDouble Beam Spectrophotometers
Double beam spectrophotometers are fast and accurate, using two beams of light—a sample beam and a reference beam—to correct instrument fluctuations in real-time.
Learn More- Key Literature
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Cary 3500 UV-Vis Brochure
Learn about the Agilent Cary 3500 UV-Vis spectrophotometer
- Brochures
- English
- 04 Sep 2024
- 4.87 MB
Amplify Confidence in Your Data Integrity
How to get the functionality you need for compliance with 21 CFR Part 11 or EU Annex 11 when using the Agilent Cary 3500 UV-Vis instrument.
- Flyers
- English
- 29 Aug 2022
- 749.43 KB
10-Year Xenon Flash Lamp Replacement Warranty - Cary 3500 UV-Vis
The Agilent Cary 3500 UV-Vis series offers flexibility in sample measurement with multiple modules. The xenon flash lamp reduces frequency and cost of lamp replacement and is covered by a 10-year warranty.
- Flyers
- English
- 11 Mar 2024
- 683.75 KB
- Application Notes
- Brochures
- Catalogs
- Flyers
- Posters
- Primers
- Technical Overviews
- White Papers
- FAQs
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UV-Vis Spectroscopy and Spectrophotometry Overview
An introduction to the basics of UV-Vis spectroscopy and UV-Vis spectrophotometers, with FAQs on the fundamentals of UV-Vis, how UV-Vis spectrophotometers work, UV-Vis analysis, applications, accessories, and measurement techniques.
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Upgrade Cary UV Workstation: Smarter Reporting, Advanced Calculations, Seamless Compliance
Upgrade to Cary UV Workstation v1.6 for smarter workflows and customizable reporting. Autogenerate PDF/CSV reports, export to custom locations, and scale graphs for clarity. Use 50+ built-in or custom equations with sample metadata. Overlay spectra for easy comparisons. Supports USP <857>, Ph. Eur. 2.2.25, JP XVIII, OpenLab 2.8, ECM 3.6, and Windows 10/11 for compliance and seamless integration.
- 08 Oct 2025
Quality Assessment of Saffron by UV Vis Spectroscopy in Accordance with ISO 3632
In this application note, the Cary 3500 Multicell UV-Vis was used to determine the concentration of key bioactive markers, crocin (carotenoid; color), picrocrocin (bittering agent; flavor), and safranal (aroma) as per ISO 3632-2:2010. The sample was graded Extra Class, as per ISO 3632-1:2025. This application note highlights the benefits of the Agilent Cary UV Workstation in simplifying the workflow, through software spectral features, supporting high-throughput environments.
- 30 Sep 2025
UV-Vis Spectroscopy for Quality Assessment of Paprika as per ASTA Method 20.1
This application note highlights benefits of using the Cary 3500 Multicell UV-Vis to commercially grade paprika quality. As per ASTA Method 20.1, two paprika samples were graded with an ASTA color value, based on quantification of carotenoid pigments. Cary UV Workstation simplified data analysis with intuitive software features that eliminated the need for manual or external data processing tools.
- 30 Sep 2025
Read reviews on the Cary 3500 UV-Vis Spectrophotometer
Explore customer reviews posted on SelectScience, or write your own review.
UV-Vis Pharmaceutical Compliance & Data Integrity
Learn about meeting UV-Vis compliance requirements, protecting data, and guaranteeing accurate results.
Thermal Melts Time Saving Calculator
See how much time the Cary 3500 UV-Vis can save your lab compared to your current thermal melt workflows.
UV-Vis Spectroscopy Learning Tools
Get your Basics of UV-Vis Spectrophotometry handbook, and a pack of four undergraduate teaching lab experiments.
Multicell UV-Vis Spectrometer Applications
Discover unique multicell UV-Vis experiment capabilities—for routine analysis and challenging measurements.
Alexandra Heussner, PhD
Laboratory Manager, Analytical Science Laboratory (ASL), Vetter Pharma-Fertigung GmbH & Co. KG, Ravensburg, Germany
There is a trend towards using melting temperature (Tm) in combination with intact mass measurement, combining sequence and mass to achieve unequivocal identification. Though different analytical techniques have been thoroughly reviewed, this Tm-based strategy has been discussed at expert conferences and is supported by many oligonucleotide-developing and -producing pharmaceutical companies. For this approach, the Cary 3500 UV‑Vis provides state-of-the-art technology. Temperature can be accurately set with the integrated Peltier element and controlled with the in-cuvette probes. The design of the multizone instrument with four cuvette pairs allows measuring samples and references in paral
View MoreCarlos Santos-Martin
PhD Candidate, La Trobe University, Australia
Carlos discusses his work on urinary tract infections, including multicell use of the Cary 3500 UV-Vis.
View MoreBenjamin Tadgell, Eser M. Akinoglu
ARC Centre of Excellence in Exciton Science School of Chemistry, University of Melbourne
We are impressed with the fast scan, user friendly modular design and high throughput of Agilent Cary 3500 UV-Vis. In particular, the great temperature ramping rate and sensitivity makes our work easier. Our project uses UV-Vis to research how temperature affects the scattering properties of gold nanoparticles coated in PNIPAM polymer. As temperature increased over the 30 – 40 °C range, the polymer collapses and the particles aggregate; we are interested in understanding the dynamics of these processes. Wavelength scans at different temperatures, and thermal gradient scans are used to partially characterize these processes. We also do these measurements over a range of pH, salt concentrat
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