Cell Analysis Technology News and Information
Measure what matters – Agilent live-cell metabolism solutions
With over 20,000 genes, 200,000 proteins and thousands of pathways, you can't measure everything in a cell at once, but you can measure the energy that drives them.
Agilent offers cell analysis solutions that detect discrete changes in cellular bioenergetics in real-time. Real-time cell measurements provide a clear window into the critical functions driving cell signaling, proliferation, activation, toxicity and biosynthesis. Move beyond analyzing what your cells are to reveal a clearer picture of what they do.
Discover the drivers of cell fate, function & fitness
Mitochondrial function and glycolysis play critical roles in a variety of cellular processes. Discover how real-time, live cell analysis can provide a complete picture of what's driving your cells' phenotype and function.
Cancer - discover drivers, dependencies and liabilities of cancer cell biology
Cancer cells reprogram their metabolism in order to generate the energy and building blocks they need to proliferate. Agilent Seahorse XF technology provides a window into the Warburg effect, fuel usage and other events that drive tumor cell biology.
Immunology – unlock immunometabolism, the driver of immune cell function
Immunometabolism has emerged as a crucial component to understand the connection between metabolic pathways and immune responses. Seahorse XF Technology enables researchers to understand and perturb immune activation and response in real-time through metabolic measurements.
Drug discovery – expand your pipeline, target novel metabolic pathways
From target identification and validation, to toxicology, and mechanism of action, being able to collect direct measurements of cell and energy metabolism will allow you to expand your drug discovery pipeline by targeting metabolic pathways, assessing compounds for mitochondrial toxicity, and better understanding their mechanisms.
Immunotherapy – tune the drivers of immune cell fate, fitness and potency
Developing immune cell therapies requires an in depth understanding of immune cell function. This requires key technologies from high-efficiency gene editing to assessment of real-time cell function, phenotype, and fate. Discover Agilent's tool bench of solutions that will enable you to develop potent and safe therapeutics.
Flexible solutions for real-time, live cell metabolic analysis
Agilent offers two platforms for studying live cell metabolism. The Seahorse XF platform is a label-free, end-to-end, integrated platform that seamlessly combines instrument, sensor cartridge, assay kits and software to deliver quantitative cell metabolic data with real-time compound exposure kinetics. The MitoXpress and pH-Xtra assays are soluble sensor reagents for TR-F enabled plate-readers.
- Seahorse XF analyzers, consumables, and software
- MitoXpress Xtra and pH-Xtra Assays for use with your microplate reader
Powerful cell energy metabolic analysis
Find the right instrument for your workflow and throughput, the right assay for your biological question and interpret results with our powerful software.
Instruments
Agilent offers three configurations of XF Instruments, depending on your throughput needs and sample type.
Assays & consumables
Easy to use assay kits, FluxPaks, media and reagents simplify Seahorse XF Analysis. Search by instrument type.
Software
Design, run and analyze assay data with our complete suite of software products.
MitoXpress Xtra and pH-Xtra assays
These assays use soluble, chemically inert, cell impermeable, fluorophores to measure OCR and ECAR in real time, using plate-reader based assays. They arewell suited for higher t hroughout assay applications, suspension cell and bacterial cell samples and can be multiplexed with other fluorescent dyes and labels.
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Already have access to a compatible plate reader? Get started measuring metabolism today!
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Find Your Plate Reader
Discover which assays are compatible with which plate readers to complete your workflow.
Selection toolEducational & planning resources
Learn how to run an XF assay
The Seahorse XF Assay Learning Center provides protocols, techniques and resources aligned to each step of the XF workflow to ensure optimal assay performance and results. The XF Assay Learning Center has been recently updated to reference the new Seahorse XF Real-Time ATP Rate Assay for performing initial cell characterization.
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Getting started
Seahorse XF Assay learning center
The XF Assay Learning Center provides the most up-to-date information on protocols and procedures for preparing, running, and analyzing an XF assay.
Understanding which Agilent cell metabolism assay best suits your biological questions, workflow, and throughput goals.
Seahorse XF instruments selection guide
All Seahorse XF instruments analyze mitochondrial respiration and glycolysis in live cells, generating data in just minutes.
Seahorse XF technology uses solid-state sensors to simultaneously measure both oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) in every well.
Make Seahorse part of your next NIH grant application
Seahorse XF is a standard for measuring functional cell metabolism that can be leveraged to support the diverse research aims within grant-funded research projects. Learn from experts and existing users how to leverage Seahorse XF to strengthen your NIH grant application.
Videos
Demonstrating how the XF Technology works and how it is being used.
Focusing on a wide range of topics.
Publication database
Cell Analysis Publications Database
Browse the full list of publications using Agilent Cell Analysis data.
Unique insights delivered by validated, end-to-end metabolic assays
Agilent has many standard assays built on oxygen and pH sensor technologies to help you to address your research needs.
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Quantify ATP production by glycolysis & OXPHOS
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Measure key parameters in mitochondrial function
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Determine glycolytic activities in live cells
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Investigate substrate/fuel dependence
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Detect intracellular oxygen
A more informative approach to assess the interplay between energy metabolism and cellular functions in response to interventions
Learn More about the Seahorse XF assay kit
The well-recognized Assay for studying mitochondrial function, providing basal respiration, ATP-linked respiration, maximal respiration and spare respiratory capacity.
Learn More about the Seahorse XF assay kit
Learn More about the plate reader compatible assay
Get a complete profile of cellular glycolysis. Quantify glycolytic rate and pathway capacity.
Learn More about the Seahorse XF assay kit
Learn More about the plate reader compatible assay link
Discover how cell phenotype or fate can be controlled via manipulation or control of substrate oxidation activities.
Learn More about the Seahorse XF assay kit
A unique, powerful tool for the quantification of real-time cellular oxygenation, revealing the interplay between cellular oxygenation and metabolism.
Learn More about the plate reader compatible assay
Community Center
Cell analysis community
Agilent community for Seahorse and Soluble Sensors:
Core facilities with Seahorse XF Research Services
Seahorse grant expeditor package can simplify your grant application process
Worldwide Technical Support
E-mail:
cellanalysis.support@agilent.com
US and Canada
1-800-227-9970; select option 3 then 8
Europe
UK: 0800 096 7632
Germany: 0800 180 66 78
Netherlands: 0800 022 7243
Other EU: +45 3136 9878
For Research Use Only. Not for use in diagnostic procedures.