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Agilent Cell Analysis Technology News and Information

Cell Analysis - Measure What's Important

With over 20,000 genes, 200,000 proteins and thousands of pathways, you can't measure everything in a cell at once, but you can measure the energy that drives them.
Agilent offers cell analysis technology solutions that detect discrete changes in cellular bioenergetics in real-time. Real-time cell measurements provide a clear window into the critical functions driving cell signaling, proliferation, activation, toxicity and biosynthesis. Move beyond analyzing what your cells are, and reveal a clearer measure of what they do.

Flexible solutions for real-time, live cell metabolic analysis

Agilent offers two platforms for studying cell metabolism. The Seahorse XF platform is a label-free, integrated platform that seamlessly combines instrument, sensor cartridge, assay kits and software to deliver quantitative cell metabolic data with real-time compound exposure kinetics. Agilent also offers soluble sensor assay kits, MitoXpress and pH-Xtra plate-reader ready options in a flexible format, ideally suited to high throughput assay applications. These plate-reader ready options are designed in a flexible format to accommodate all low to high throughput assay applications.

Wide Selection of Assay Kits

Agilent has many standard assays built on oxygen and pH sensor technologies to help you to address your research needs.
Discover how you can gain insights in your cell function through:
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Measure ATP production rates from glycolysis and mitochondrial respiration in live cells. Quantify metabolic switching.

The gold standard in mitochondrial functionality, providing basal respiration, ATP-linked respiration, maximal respiration and spare respiratory capacity.

Get a complete profile of cellular glycolysis. Quantify glycolytic rate and pathway capacity.

Understand cell fuel/substrate metabolism from three fuel sources. Identify preference, flexibility and fuel liability.

A unique, powerful tool for the quantification of real-time cellular oxygenation, revealing the interplay between cellular oxygenation and metabolism.

Learn how to run an XF assay

The Seahorse XF Assay Learning Center provides protocols, techniques and resources aligned to each step of the XF workflow to ensure optimal assay performance and results. The XF Assay Learning Center has been recently updated to reference the new Seahorse XF Real-Time ATP Rate Assay for performing initial cell characterization.

Educational Resources

Seahorse XF Assay Learning Center
The XF Assay Learning Center provides the most up-to-date information on protocols and procedures for preparing, running, and analyzing an XF assay.
Cell Analysis Selection Guide
Understanding which Agilent cell metabolism assay best suits your biological questions, workflow, and throughput goals.
Seahorse XF Instruments Selection Guide
All Seahorse XF instruments analyze mitochondrial respiration and glycolysis in live cells, generating data in just minutes.
How Seahorse Analyzers Work
Seahorse XF technology uses solid-state sensors to simultaneously measure both oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) in every well.
Cell Analysis videos
Demonstrating how the XF Technology works and how it is being used.
Cell Analysis webinars
Focusing on a wide range of topics.
Cell Analysis Publications Database
Browse the full list of publications using Agilent Cell Analysis data.

Powerful Cell Energy Metabolic Analysis

Find the right instrument for your workflow and throughput, the right assay for your biological question and interpret results with our powerful software.
Agilent offers three configurations of XF Instruments, depending on your throughput needs and sample type.
Assays & Consumables
Easy to use assay kits, FluxPaks, media and reagents simplify Seahorse XF Analysis. Search by instrument type.
Design, run and analyze assay data with our complete suite of software products.

Solutions for your applications

Mitochondrial function and glycolysis play critical roles in a variety of cellular processes, including cellular activation, proliferation, differentiation, cell death, and disease progression. Discover how real-time, live cell analysis can provide a complete picture of what's driving your cells' phenotype and function.

Cancer Research

Cancer cells reprogram their metabolism in order to generate the energy and building blocks they need to proliferate. Agilent Seahorse XF technology provides a window into the Warburg effect, fuel usage and other events that drive tumor cell biology.

Immunology Research

Immunometabolism has emerged as a crucial component to understand the connection between metabolic pathways and immune responses. Seahorse XF Technology enables researchers to understand and perturb immune activation and response in real-time through metabolic measurements.

Drug Discovery

From target identification and validation, to toxicology, and mechanism of action, being able to collect direct measurements of cell and energy metabolism will allow you to expand your drug discovery pipeline by targeting metabolic pathways, assessing compounds for mitochondrial toxicity, and better understanding their mechanisms.

Obesity, Diabetes and Metabolic Disorders

Research into metabolic profiles and changes is enabling insight into browning, substrate and nutrient utilization, and inflammation. Agilent Seahorse XF technology provides the capability to examine functional metabolism in models of cellular metabolic syndromes.

Neurobiology Research

Researchers are using Agilent Seahorse XF technology to explore metabolic changes in neuronal systems, using live cells, in real-time, requiring less biological samples than traditional assays.

Stem Cell Research

Metabolic shift in induced pluripotent stem cells (iPSCs), has a significant importance in establishing pluripotent identity. Using Agilent Seahorse XF technology, researchers can predict the ability of somatic cell reprogramming to iPSCs, and how PSCs alter their metabolism during the differentiation process to terminally differentiated cells.

Immunotherapy Research

Developing immune cell therapies requires an in depth understanding of immune cell function. This requires key technologies from high-efficiency gene editing to assessment of real-time cell function, phenotype, and fate. Discover Agilent's tool bench of solutions that will enable you to develop potent and safe therapeutics.


Community Center

Core Facilities with Seahorse XF Research Services.
Cell Analysis Travel Awards for graduate students and young investigators.
Seahorse Grant Expeditor Package can simplify your grant application process.
Worldwide Technical Support
USA and Canada
1-800-227-9770; select option 3 then 8
UK: 0800 096 7632
Germany: 0800 180 66 78
Netherlands: 0800 022 7243
Other EU: +45 3136 9878
For Research Use Only. Not for use in diagnostic procedures.