With over 20,000 genes, 200,000 proteins and thousands of pathways, you can't measure everything in a cell at once, but you can measure the energy that drives them.
Agilent offers cell analysis technology solutions that detect discrete changes in cellular bioenergetics in real-time. Real-time cell measurements provide a clear window into the critical functions driving cell signaling, proliferation, activation, toxicity and biosynthesis.
Move beyond analyzing what your cells are, and reveal a clearer measure of what they do.
Flexible solutions for real-time, live cell metabolic analysis
Agilent offers two platforms for studying cell metabolism.
Seahorse XF platform is a label-free, integrated platform that seamlessly
combines instrument, sensor cartridge, assay kits and software to deliver
quantitative cell metabolic data with real-time compound exposure kinetics.
Agilent also offers soluble sensor assay kits,
MitoXpress and pH-Xtra
plate-reader ready options in a flexible format,
ideally suited to high throughput assay applications.
These plate-reader ready options are designed in a flexible format to
accommodate all low to high throughput assay applications.
Measure ATP production rates from glycolysis and mitochondrial respiration in live cells. Quantify metabolic switching.
The gold standard in mitochondrial functionality, providing basal respiration, ATP-linked respiration, maximal respiration and spare respiratory capacity.
Get a complete profile of cellular glycolysis. Quantify glycolytic rate and pathway capacity.
Understand cell fuel/substrate metabolism from three fuel sources. Identify preference, flexibility and fuel liability.
A unique, powerful tool for the quantification of real-time cellular oxygenation, revealing the interplay between cellular oxygenation and metabolism.
Learn how to run an XF assay
The Seahorse XF Assay Learning Center provides protocols, techniques and resources aligned to each step of the XF workflow to ensure optimal assay performance and results. The XF Assay Learning Center has been recently updated to reference the new Seahorse XF Real-Time ATP Rate Assay for performing initial cell characterization.
Mitochondrial function and glycolysis play critical roles in a variety of cellular processes, including cellular activation, proliferation, differentiation, cell death, and disease progression. Discover how real-time, live cell analysis can provide a complete picture of what's driving your cells' phenotype and function.
Cancer cells reprogram their metabolism in order to generate the energy and building blocks they need to proliferate. Agilent Seahorse XF technology provides a window into the Warburg effect, fuel usage and other events that drive tumor cell biology.
Immunometabolism has emerged as a crucial component to understand the connection between metabolic pathways and immune responses. Seahorse XF Technology enables researchers to understand and perturb immune activation and response in real-time through metabolic measurements.
From target identification and validation, to toxicology, and mechanism of action, being able to collect direct measurements of cell and energy metabolism will allow you to expand your drug discovery pipeline by targeting metabolic pathways, assessing compounds for mitochondrial toxicity, and better understanding their mechanisms.
Research into metabolic profiles and changes is enabling insight into browning, substrate and nutrient utilization, and inflammation. Agilent Seahorse XF technology provides the capability to examine functional metabolism in models of cellular metabolic syndromes.
Metabolic shift in induced pluripotent stem cells (iPSCs), has a significant importance in establishing pluripotent identity. Using Agilent Seahorse XF technology, researchers can predict the ability of somatic cell reprogramming to iPSCs, and how PSCs alter their metabolism during the differentiation process to terminally differentiated cells.