ZORBAX Reversed-Phase HPLC Columns with new sustainable packaging
ZORBAX Reversed-Phase HPLC Columns
ZORBAX is one of the most popular high-performance reversed-phase HPLC column families. ZORBAX columns are based on traditional fully porous particles and offer the highest loading capacity and resolution. From Eclipse Plus, Eclipse XDB, StableBond, Bonus-RP and more, the ZORBAX column portfolio offers a wide selection of stationary phases allowing you to fine-tune your selectivity to match your application. Improve resolution by utilizing sub-2 µm particles to maximize system efficiency and confidently scale-up to preparative HPLC and/or easy HPLC method transfer.
Product Details
Features
- Traditional, fully porous particles available in 1.8, 3.5, and 5 µm sizes. With selected products also available in 7 μm particles for preparative HPLC (see Prep LC columns section)
- ZORBAX Eclipse Plus columns are the recommended starting point for method development at mid-pH and benefit from the ultimate performance from fully porous silica-based columns
- ZORBAX StableBond columns offer the highest stability for low pH applications down to pH 1 while ZORBAX SB-Aq deliver robust, reproducible separations with highly aqueous mobile phases, including 100% water
- ZORBAX Eclipse XDB columns – C18, C8, Phenyl and CN – provide four bonded phase choices for method development optimization. These columns are eXtra Densely Bonded (XDB) and double endcapped through a proprietary process to cover as many active silanols as possible. Eclipse XDB columns are available in 1.8, 3.5, 5 and 7 μm particle sizes for high speed, high resolution, analytical and prep scale separations
- ZORBAX Extend-C18 columns offer high efficiency and selectivity advantages for basic compounds in for high pH applications up to pH 11.5
- ZORBAX Bonus RP columns offer unique selectivity due to an embedded amide linkage in the C14-alkyl chain making them ideal for 100% aqueous mobile phases and for compounds that are not retained on standard reverse phase columns
- ZORBAX Rx columns are made from highly pure (>99.995 %) porous silica with a higher carbon load (12%)
- ZORBAX Eclipse PAH columns deliver high resolution separation of the 16 polycyclic aromatic hydrocarbons (PAHs) in EPA 610 and have been specifically tested with PAHs for maximum reproducibility
- ZORBAX Rapid Resolution High Definition (RRHD) columns are packed with 1.8 μm particles and stable up to 1200 bar for fast, high-resolution separations of your most complex samples
- ZORBAX Rapid Resolution High Throughput (RRHT) columns use totally porous 1.8 μm particles to provide maximum resolution in ultra-fast and high-resolution analyses with a pressure limit of 600 bar
- Agilent UHPLC Guards extend the lifetime of analytical columns - without diminishing performance - and use easy-to-install hardware that fits directly on the end of the column
How It Works
Applications
Featured References
- Publications
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- Wongchang, T.; Winterberg, M.; Tarning, J.; Sriboonvorakul, N.; Muangnoicharoen, S.; Blessborn, D. Determination of ceftriaxone in human plasma using liquid chromatography-tandem mass spectrometry. Wellcome Open Research 2022, 4, 47. DOI: 10.12688/wellcomeopenres.15141.3.
- Islam, S. F.; Kabir, M. H. M.; Yasmin, S. Y. S.; Alam, M. J.; Ahmed, S. A. S.; Rahman, M. S. A modified QuEChERS method development to analyze tylosin and metronidazole antibiotics residue in shrimp (Penaeus monodon) using LC-ESI MS/MS. Frontiers in Sustainable Food Systems 2023, 7.
- Hori, M.; Nakazawa, H. Determination of trenbolone and zeranol in bovine muscle and liver by liquid chromatography-electrospray mass spectrometry. Journal of Chromatography A 2000, 882 (1–2), 53–62. DOI: 10.1016/S0021-9673(00)00205-3.
- Bermingham, S.; O'Connor, R.; Regan, F.; McMahon, G. P. Simultaneous determination of anthracyclines and taxanes in human serum using online sample extraction coupled to high performance liquid chromatography with UV detection. Journal of Separation Science 2010, 33 (11), 1571–1579. DOI: 10.1002/jssc.201000026.
- Pous-Torres, S.; Torres-Lapasió, J. R.; García-Álvarez-Coque, M. C. Comparison of the performance of Chromolith Performance RP-18e, 1.8-μm Zorbax Eclipse XDB-C18 and XTerra MS C18, based on modelling approaches. Analytical and Bioanalytical Chemistry 2013, 405 (7), 2219–2231. DOI: 10.1007/s00216-012-6448-y.
- Lin, F.; An, Y.; Mei, F.; Xia, T.; Jiang, H.; Peng, L.; Qiao, W.; Guo, D.-A. Quantitative Evaluation of the Quality of Shiqi Waigan Granules Based on Fingerprinting and Multi-Indicator Components With Multivariate Statistical Analysis. Biomedical Chromatography 2025, 39 (11), e70221. DOI: 10.1002/bmc.70221.
- Dommarco, R.; Santilio, A.; Fornarelli, L.; Rubbiani, M. Simultaneous quantitative determination of thirteen urea pesticides at sub-ppb levels on a Zorbax SB-C18 column. Journal of Chromatography A 1998, 825 (2), 200–204. DOI: 10.1016/S0021-9673(98)00728-6.
- Chinta, S. R.; Chintala, V.; Nandimalla, V.; Ch, R.; Ediga, S. G.; Kowtharapu, L. P.; Katari, N. K. Quantification of Drospirenone- and Ethinyl Estradiol-Related Impurities in a Combined Pharmaceutical Dosage Form by a Chromatography Method With a QbD Robustness Study. Journal of AOAC International 2024, 107 (1), 31–39. DOI: 10.1093/jaoacint/qsad118.
- Yu, Y.; Yang, Y.-Q.; Zeng, N.-J.; Zhang, H.-W.; Sun, L.-N.; Wang, Y.-Q. Simultaneous Determination of Voriconazole and Its Voriconazole N-Oxide Metabolite in Human Urine by Liquid Chromatography/Tandem Mass Spectrometry. Journal of Chromatographic Science 2022, 60 (8), 800–806. DOI: 10.1093/chromsci/bmab126.
- Qian, Z. M.; Wan, J. B.; Zhang, Q. W.; Li, S. P. Simultaneous determination of nucleobases, nucleosides and saponins in Panax notoginseng using multiple columns high performance liquid chromatography. Journal of Pharmaceutical and Biomedical Analysis 2008, 48 (5), 1361–1367. DOI: 10.1016/j.jpba.2008.09.038.
- Ruiz-Angel, M. J.; Berthod, A. Reversed-phase liquid chromatography analysis of alkyl-imidazolium ionic liquids II. Effects of different added salts and stationary phase influence. Journal of Chromatography A 2008, 1189 (1–2), 476–482. DOI: 10.1016/j.chroma.2007.10.046.
- Lindegårdh, N.; Blessborn, D.; Bergqvist, Y. Simultaneous Quantitation of the Highly Lipophilic Atovaquone and Hydrophilic Strong Basic Proguanil and Its Metabolites Using a New Mixed-Mode SPE Approach and Steep-Gradient LC. Journal of Chromatographic Science 2005, 43 (5), 259–266. DOI: 10.1093/chromsci/43.5.259.
- Houbenová, A.; Claessens, H. A.; de Haan, J. W.; Cramers, C. A.; Stulík, K. An investigation concerning the stability of a sterically protected cyanopropyl modified silica substrate. Journal of Liquid Chromatography 1994, 17 (1), 49–68. DOI: 10.1080/10826079408013435.
- Qian, Z. M.; Wan, J. B.; Zhang, Q. W.; Li, S. P. Simultaneous determination of nucleobases, nucleosides and saponins in Panax notoginseng using multiple columns high performance liquid chromatography. Journal of Pharmaceutical and Biomedical Analysis 2008, 48 (5), 1361–1367. DOI: 10.1016/j.jpba.2008.09.038.
- Pelletier, S.; Lucy, C. A. Fast and high-resolution ion chromatography at high pH on short columns packed with 1.8 μm surfactant coated silica reverse-phase particles. Journal of Chromatography A 2006, 1125 (2), 189–194. DOI: 10.1016/j.chroma.2006.05.059.
- Otaegui, D.; Masdeu, C.; Aldaba, E.; Vara, Y.; Zubia, A.; San Sebastian, E.; Alcalá, M.; Villafruela, S.; Cossío, F. P.; Rodriguez-Gascón, A. Development and validation of a LC-MS assay for the quantification of ikh12 a novel anti-tumor candidate in rat plasma and tissues and its application in a pharmacokinetic study. Biomedical Chromatography 2015, 29 (8), 1249–1258. DOI: 10.1002/bmc.3414.
- Liu, A.-H.; Li, L.; Xu, M.; Lin, Y.-H.; Guo, H.-Z.; Guo, D.-A. Simultaneous quantification of six major phenolic acids in the roots of Salvia miltiorrhiza and four related traditional Chinese medicinal preparations by HPLC-DAD method. Journal of Pharmaceutical and Biomedical Analysis 2006, 41 (1), 48–56. DOI: 10.1016/j.jpba.2005.10.021.
- Anspach, B.; Gierlich, H. U.; Unger, K. K. Comparative study of Zorbax Bio Series GF 250 and GF 450 and TSK-Gel 3000 SW and SWXL columns in size-exclusion chromatography of proteins. Journal of Chromatography 1988, 443, 45–54. DOI: 10.1016/S0021-9673(00)94781-2.