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One of the most diversified reversed-phase LC and HPLC column portfolios

Reversed-phase LC, the most popular separation mode in liquid chromatography, can be used for many different separation tasks. To achieve the best separation results, the right reversed-phase column choice is of utmost importance. Columns are available in many different stationary phases to be used in ultra-high-performance liquid chromatography (UHPLC) and high-performance liquid chromatography (HPLC), and deliver exceptional performance with outstanding lifetime and robustness. Reversed-phase HPLC columns, including Zorbax and InfinityLab Poroshell, are designed and manufactured end-to-end by Agilent. The column manufacturing process includes multiple Quality Control (QC) tests so the chromatographer is assured of rugged, reproducible performance with long column lifetime. Reversed-phase HPLC columns include InfinityLab Poroshell 120, Zorbax, Pursuit, XRs and XRs Ultra, Polaris, and HC-C18(2) and TC-C18(2).

Reversed-Phase LC Columns | Liquid Chromatography

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Featured Tools

HPLC & LC Column Selector Tool

A series of tools to help you navigate to the best HPLC column choice for your application.

Preparative HPLC Scaling Calculator

Scale between analytical and prep methods; calculate column capacity, # of injections, run time & mobile phase use.

Bio HPLC Columns eSelector Tools

Intuitive process to help you select the right chromatography column for your biopharmaceutical applications.

Essential Supplies Guide for InfinityLab LC Series Instruments

Discover the right supplies for your instrument to achieve peak performance and optimize your LC workflow.

Featured Literature

More Chemistries, More Choices For Solving our Toughest Separation Challenges

The InfinityLab Poroshell 120 family has grown to include 3 particle sizes and 19 chemistries, so you can efficiently separate the widest variety of compounds.

Scale up to the Fast Lane with InfinityLab Poroshell 120 Prep Columns

Is your purification lab sacrificing speed to find confident answers to research questions?

A Proven and Reliable Portfolio of Totally Porous HPLC Columns

The Agilent ZORBAX family offers all advantages of totally porous particle columns such as increased retention, loadability and resistance to sample solvents.

Featured Customer Testimonials

Dr. Stefan Bieber

Managing Director, AFIN-TS GmbH

Our SFC method development strategy always includes stationary phases of Agilent Technologies, Inc. We strongly appreciate the robustness of polar ZORBAX and Poroshell phases, such as ZORBAX Rx-SIL and Poroshell 120 HILIC. They exhibit good selectivity and high separation performance, which is key for successful and efficient method development in SFC. The broad spectrum of available column dimensions has great advantage for the optimization of separations and allows us to adjust the column dimension to obtain the most efficient separations.

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Sébastien Leclerc

Analytical Chemist, SANEXEN Environmental Services, Inc.

The partnership with Agilent extends way beyond the initial purchase or initial installation. They provide the consumables and also the expertise.

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Featured News

Per- and Polyfluoroalkyl Substances (PFAS) Analysis For Environmental Samples

Agilent offers complete end-to-end workflows for extraction, quantification even at Ultra-trace levels, and reporting of PFAS in the environment. This includes a range of sample preparation products, HPLC columns, chemical standards, PFC-free sample containment and other HPLC supplies, Ultra-high performance liquid chromatography (UHPLC) coupled to triple quadrupole mass spectrometry, e-methods, as well as gas chromatography products for sampling and analyzing a challenging range of trace-level volatile and semivolatile vapors of PFAS in air.

Develop Fast Purification Methods for Natural Products

A fast and simple purification method for withaferin A in ashwagandha extract was successfully developed using an Agilent InfinityLab Poroshell 120 SB-C18 preparative HPLC column. The InfinityLab Poroshell 120 column provided better resolution and a 45% decrease in run time over a traditional preparative column. The performance benefits of InfinityLab Poroshell preparative columns, especially at high flow rates, align well with the needs of pharmaceutical discovery laboratories and other high-throughput environments.

Full Workflow for US EPA Method 1633 for Targeted PFAS Analysis

Agilent announced a highly-anticipated, complete workflow solution for targeted per- and polyfluoroalkyl substances (PFAS) analysis using the United States (US) Environmental Protection Agency (EPA) Method 1633 (3rd draft). EPA Draft Method 1633 currently analyzes 40 PFAS compounds in wastewater and soil and is a complex and labor-intensive method, relying on multiple sample preparation and analysis steps. Success in running the technique depends on careful sample handling and the appropriate sample preparation supplies and instrumentation.

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