ZORBAX 300Å StableBond columns are ideal for the reproducible separations of proteins and peptides. The wide, 300Å pore on these reversed-phase HPLC columns is essential for an efficient separation of these large biomolecules, in order for them to completely access the bonded phase. The ZORBAX RRHD columns have 1.8 µm particles which are compatible with UHPLC and ideal for higher resolution separations with shorter run times. StableBond columns are available in four different bonded phases (C18, C8, C3, and Diphenyl) for selectivity and recovery optimization of proteins and polypeptides. To further increase sample recovery and improve efficiency for difficult proteins, 300StableBond columns can be used up to 80°C. 300SB-C18 and 300SB-C8 columns are an ideal choice for complex protein and protein digest separations. ZORBAX RRHD 300-HILIC columns are also available for glycopeptide analysis, which enable you to resolve peptide glycoforms.
This application note describes a workflow for assessing critical quality attributes (CQAs) of intact adeno-associated viruses (AAVs), together with post-translational modification (PTM) identification of the capsid proteins.
User guides contain helpful information to ensure the best performance from your column, such as operating parameters, cleaning and storage guidelines, usage tips, and suggested starting conditions.