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Agilent Seahorse XF Hu T Cell Activation Assay Kit

Rapid, Real-Time Detection of ​T Cell Activation​

Immuno-metabolism is well understood to influence many aspects of immune cell response as both an indicator and controller of immune cell fate, function, and fitness.​

This concept is clearly illustrated in the metabolic ramping of naive T cells to proliferate following activation signaling, and distinct metabolic phenotypes that drive effector and memory function and T cell persistence.​

T Cell Activation

The new Agilent Seahorse XF Hu T Cell Activation Assay Kit is an optimized turnkey solution that detects T cell activation in minutes, providing a kinetic readout of T cell activation dynamics in real-time. This delivers a unique, early window of T cell biology, enabling researchers to quantify activation kinetics and profile modulators of T cell behavior.

Understand and control T cell activation, differentiation, and proliferation

Glucose metabolism is recognized as a driver for T cell fate and function. A rapid increase in glycolysis is a signature early event during T cell activation and is required for subsequent T cell proliferation and differentiation. By measuring the increase in glycolysis as proton efflux rate (PER), Agilent Seahorse technology provides an early kinetic window to interrogate
T cell activation.

A turnkey solution for interrogating human T cell activation
Seahorse XF Hu T Cell Activation Assay kit
Seahorse XF Hu T Cell Activation Assay Kit
XF FluxPak with PDL-coated microplates
XF FluxPak with
PDL-coated microplates
Cloud-based​ Seahorse Analytics
Cloud-based​ Seahorse Analytics
Seahorse ​XF Analyzers
Seahorse ​XF Analyzers

Agilent and STEMCELL​ Technologies have partnered to deliver an optimal solution for interrogating and modulating T cell activation on the Seahorse XF platform – the Agilent Seahorse XF Hu T Cell Activation Assay Kit.​​

The kit contains Agilent-validated ImmunoCult Human CD3/CD28 T Cell Activator and 2-deoxy-D-glucose (2-DG). ​

The immunoCult Human CD3/CD28 T Cell Activator from STEMCELL​ Technologies is a soluble tetrameric complex of human CD3/CD28 T cell ligand activators. It is easy-to-use and compatible with specially designed injection ports on Seahorse XF sensor cartridges, reducing assay preparation time and improving assay reliability.​

The ready-to-use Seahorse XF PDL-coated cell culture microplates facilitate attachment of T cells onto the well bottom, providing convenience by eliminating the time and labor involved in manually coating microplates. They also reduce data variation commonly caused by a manual coating process.​

These products are offered in 96-well and 8-well formats as well as bundled with the Seahorse XF Hu T Cell Activation Assay Kit.​

Agilent Seahorse Analytics is a web-based software platform that provides a simple, streamlined data analysis workflow for analyzing the XF T cell activation​ assay results.​​

Using this tool, Seahorse XF T cell activation kinetic data can be processed and transformed into organized, shareable summary reports and graphs in minutes.

The Agilent Seahorse XF Hu T Cell Activation Assay Kit is compatible with Seahorse XFe/XF96, XF HS Mini, and XFp Analyzers.​

The new Agilent Seahorse XF HS Mini analyzer offers improved performance, streamlined analytics, and improved immune cell workflow.

Application note

Dive into the experimental strategy, understand activation potential and quantification options, and see a demonstration of utility in assessing acute effect of immune cell modulators. ​

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Seahorse XF Hu T Cell
Activation Assay Kit
Seahorse XF Hu T Cell Activation Assay Kit

Check out the Agilent turnkey solution for early detection and real-time modulation of human T cell activation using XF analyzers.​

Seahorse XF HS Mini
Seahorse HS Mini Analyzer​

Don’t have a Seahorse XF Analyzer? Explore the latest and greatest Agilent XF technology.

Seahorse XFe96
Seahorse XFe96 Analyzer​

Need more throughput? Check out the Agilent’s XFe96 Analyzer.​

Explore other real-time activation applications with other immune cell types​
Neutrophil activation
Real-time analysis of in situ activated neutrophil
Neutrophil activation

Direct Detection of Neutrophil Oxidative Burst in Real-Time​

Neutrophils are the initial immune defense to eliminate infections, and they involve several mechanisms including the production of reactive oxygen species (ROS). Unlike conventional fluorescence or luminescence detection methods, Agilent Seahorse XF technology enables real-time detection of neutrophil activation by simultaneously measuring the oxygen consumption rate (OCR) and proton efflux rate (PER). ​

Discover how the Agilent Seahorse XF platform is used to study the innate immune responses to modulators, pharmaceutical interventions, and microenvironment impact.​

- Download poster

- Download application note

- Watch webinar

- Data analysis tutorial

Macrophage activation
Real-time analysis of in situ activated macrophages
Macrophage activation

Measure and Discriminate Macrophage Activation in Real-Time​

Glycolytic pathway dependency for energy production is a robust measure of macrophage activation. Unlike conventional end-point assays, which are used to measure macrophage activation in terms of changes in cytokine expression, the Agilent Seahorse XFp Analyzer detects macrophage activation response by measuring the proton efflux rate (PER) in real-time, providing an early window of functional information to discriminate activation responses. ​

Discover how the noninvasive, real-time Agilent Seahorse XF platform discriminates macrophage activation within two hours in a standardized approach. ​​

- Download poster

- Download application note

- Watch webinar

Reference our publication database for a full listing of relevant immunology relevant publications using Seahorse XF technology, here. ​