Seahorse XF Glycolysis Stress Test Kit
The Glycolysis Stress Test kit contains the materials sufficient for six full plate tests and is available in two packaging sizes. Each kit contains the material sufficient for six full plate tests. The XF kit is for use with 96- or 24-well Seahorse Analyzers. The XFp kit is for use with 8-well Seahorse Analyzers.
- XF Assay Kits, Reagents & Cell Assay Media
Product Details
- The assay measures basal glycolysis and glycolysis capacity upon blockage of mitochondrial ATP production using respiratory modulators
- Non-glycolytic acidification is assessed through glucose starvation
- Compounds are reconstituted directly in the assay medium, eliminating the need for DMSO or other solvents
- Each lot is rigorously tested and validated with functional assays to deliver consistent and robust results, eliminating the cost associated with failed experiments
- The Agilent Seahorse Report Generator simplifies data processing by automatically calculating the key parameters of your samples
Automatic measurement of energy metabolism in real time
Agilent Seahorse XF Analyzers measure oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a multi-well plate, interrogating key cellular functions such as mitochondrial respiration and glycolysis. Seahorse XF Analyzers perform compound addition and mixing, label-free analytical detection, and automatic measurement of OCR and ECAR in real time.
Learn More- Application Notes
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Identifying Metabolic Switches Using an Agilent Seahorse XFe Analyzer in Hypoxia
Identifying Metabolic Phenotype Switches in Cancer Cells Using the Agilent Seahorse XF/XFe Analyzer in an Hypoxic Environment
- Application Notes
- English
- 03 Jan 2017
- 744.28 KB
Measuring glycolytic function in cells
Real-time, kinetic measurement of glycolysis connects energy pathways to cell growth and proliferation
- Application Notes
- English
- 08 Mar 2017
- 1.07 MB
Measuring glycolysis and oxidative metabolism in cancer cells
Real-time assay enables scientists to connect metabolic pathways with oncogenic phenotypes
- Application Notes
- English
- 07 Mar 2017
- 721.04 KB
Assessing the impact of autophagy on cellular metabolism
Real-time, functional metabolic assays connect energy pathways and autophagy
- Application Notes
- English
- 22 Dec 2016
- 1.06 MB
- Flyers
- Selection Guides
Agilent Cell Analysis Publication Database
Search scientific publications by research area, cell type and more.
Seahorse XF Assay Learning Center
Up-to-date information on protocols and procedures for preparing, running, and analyzing an XF assay.
Agilent Cell Analysis Webinars
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The Agilent Seahorse XF Pro platform
Transforming Bioenergetics - Again. Featuring new pharma-oriented workflow solutions with improved precision, along with advanced experimental design and analysis tools, you can optimize your workflow from start to finish.
- 17 Jan 2022
The Seahorse XF Flex Analyzer: Powerful Metabolic Insights in 2D & 3D Models
The 24-well Agilent XF Flex Analyzer offers enhanced sensitivity, a broad detection range, and compatibility with 3D models like organoids, tissues, and co-cultures. Purpose-built consumables and powerful analysis tools help you design smarter experiments, extract deeper insights, and generate translatable data—empowering your research to reach new heights in cellular metabolism and function.
- 14 Apr 2025
- Material Safety Data Sheets
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Seahorse XF Glycolysis Stress Test Kit, Part Number 103020-100
Material Safety Data Sheet
- Material Safety Data Sheets
- English
- 08 May 2024
- 141.67 KB
Seahorse XFp Glycolysis Stress Test Kit, Part Number 103017-100
Material Safety Data Sheet
- Material Safety Data Sheets
- English
- 10 Jan 2024
- 136.46 KB
- User Manuals