Imaging Plate Reader with Upright Microscope, BioTek Cytation 7

Features

Sophisticated imaging with both upright and inverted microscopy optics offers a range of cellular and reflected light applications, yielding information in many imaging modes
Inverted microscopy module supports fluorescence, brightfield, and color brightfield to analyze both large objects and intracellular detail and enables a broad range of applications
Combination of hybrid plate reader and advanced microscopy mode provides flexible operation ready for any assay, saves time, and increases productivity in the laboratory
Multimode detection plus imaging saves time and data storage
Automation of imaging acquisition, processing, image analysis, and object count reduces manual intervention
Gen5 software facilitates powerful ROI identification, samples may be selected for high magnification and the process of imaging ROIs in batches can be accelerated for faster throughput
Take3 plate or Take3 Trio allows analysis of 16 or 48 samples in a single run, saving time compared to single-sample devices
Increased sensitivity with large bandwidth settings and increased specificity with small bandwidth settings enhance assay performance and flexibility for applications

Specifications


Automation Compatibility	BioSpa BioStack 3rd-party
Camera	Sony CMOS, 16-bit grayscale
Environment Control	4-Zone Incubation to 45 °C Condensation Control Peltier Cooling CO2, O2 Control
Imager/Reader Options	Joystick stage control Laser autofocus Reagent injectors
Imaging Methods	Montage Multi-color Single-color Time lapse Z-stacking
Imaging Modes	Widefield fluorescence Brightfield High contrast brightfield Color brightfield
Magnification Range	1.25x to 60x air
Microscope Type	Inverted microscope Upright microscope Add Inverted microscope (upgrade option)
Monochromator Detection Modes	UV-Vis Absorbance Fluorescence Intensity (variable bandwidth) Luminescence
Shaking	Linear Orbital Double-orbital
Supported Labware	6 to 1536-well plates Slides Cell culture dishes Cell culture flasks (T25) Hemocytometers Chamber slides Take3/Take3 Trio

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How It Works

Applications

Technology

Variable bandwidth for sensitivity and specificity

The Cytation plate reader optics use a quad monochromator design with variable bandwidth. Large bandwidth settings provide increased sensitivity and lower limits of detection. Small bandwidth settings deliver increased specificity with multiple signals, reducing signal cross-talk and enhancing assay performance.

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Patented laser autofocus

Laser autofocus and image-based (or software-based) autofocus are two methods of autofocus widely used in microscopy.

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Time-resolved fluorescence

Time-resolved fluorescence (TRF) measurement is very similar to fluorescence intensity (FI) measurement. The only difference is the timing of the excitation / measurement process.

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TR-FRET

Time-resolved fluorescence energy transfer (TR-FRET) works on the principles that when suitable pairs of fluorophores are in close proximity of one another, excitation of a lanthanide donor fluorophore results in energy transfer to an acceptor fluorophore.

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Cell Counting

Accurate cell counting is a critical step across a spectrum of cell biology applications. Direct cell counting uses methods that identify and enumerate individual cells, as opposed to indirect methods that estimate population sizes using biochemical methods.

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Object Tracking

With the incorporation of the object tracking module, available within Agilent BioTek Gen5 software for imaging and microscopy, this can be easily accomplished in a fully automated fashion.

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Automatic Region of Interest Identification

This process enables walk-away slide scanning for four or more slides, if combined with Agilent automation offerings, and can be used with the Agilent BioTek Lionheart automated microscopes and Agilent BioTek Cytation imaging multimode readers.

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Literature

Key Literature