PLRP-S HPLC Columns new sustainable packaging

PLRP-S for Biomolecules HPLC Columns

The PLRP-S media is available in a range of pore sizes and particle sizes all with identical chemistry and retention characteristics, making it ideal for small molecule, synthetic biomolecule, and macromolecule purification.

The inherently hydrophobic chemistry of the polystyrene/divinylbenzene (PS/DVB) particles means there is no bonded phase required for reversed-phase separations, which makes it a highly reproducible material that is also free from silanols and heavy metal ions. Within the extensive product range are columns suitable for analytical separations, and preparative purifications. Their thermal and chemical stability makes them ideal for purifications that require extreme conditions for sample preparation, compound elution, and column regeneration.

With a wide choice of pore sizes and extended range of operating conditions, PLRP-S provides more options to achieve the optimum separations. Particle sizes range from 3 μm to 50 μm for scale-up from the μg/mg discovery stage to multi-kg cGMP production. Excellent chemical stability, up to 1 M NaOH, permits sanitation and regeneration that increase column lifetime. PLRP-S media batch sizes of up to 600 L are available, providing single batch packing of multiple columns.

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Filter by Inner Diameter (ID) 1.0 mm 1 mm 2.1 mm 3 mm 4.6 mm 7.5 mm 25 mm 50 mm 100 mm Length 5 mm 25 mm 50 mm 100 mm 150 mm 250 mm 300 mm Particle Size 3 µm 5 µm 8 µm 10 µm 20 µm 30 µm 50 µm Pore Size 100 Å 300 Å 1000 Å 4000 Å Hardware Bulk Media PEEK Lined SS SS Pressure Rating 103 bar 207 bar 275 bar pH Range 1-14

					QTY
PL1312-3300	PLRP-S 100Å, 1.0 x 150 mm, 3 µm	3 µm	100 Å	1 Each	Quantity
PLRP-S 100Å, 1.0 x 150 mm, 3 µm. Reversed-phase HPLC column with fully porous PS/DVB particle support stable at elevated temperatures and a wide pH range(1-14). Available in range of particle and pore sizes for targeted applications Pressure Rating: 275 bar Particle Type: Fully Porous Hardware: SS Inner Diameter (ID): 1 mm Length: 150 mm Particle Size: 3 µm Pore Size: 100 Å See all product information
PL1312-1300	PLRP-S 100Å, 1.0 x 50 mm, 3 µm	3 µm	100 Å	1 Each	Quantity
PLRP-S 100Å, 1.0 x 50 mm, 3 µm. Reversed-phase HPLC column with fully porous PS/DVB particle support stable at elevated temperatures and a wide pH range(1-14). Available in range of particle and pore sizes for targeted applications Pressure Rating: 207 bar Particle Type: Fully Porous Hardware: SS Inner Diameter (ID): 1.0 mm Length: 50 mm Particle Size: 3 µm Pore Size: 100 Å See all product information
PL1312-1500	PLRP-S 100Å, 1.0 x 50 mm, 5 µm	5 µm	100 Å	1 Each	Quantity
PLRP-S 100Å, 1.0 x 50 mm, 5 µm. Reversed-phase HPLC column with fully porous PS/DVB particle support stable at elevated temperatures and a wide pH range(1-14). Available in range of particle and pore sizes for targeted applications Pressure Rating: 207 bar Particle Type: Fully Porous Hardware: SS Inner Diameter (ID): 1.0 mm Length: 50 mm Particle Size: 5 µm Pore Size: 100 Å See all product information
PL1412-6100	PLRP-S 100Å, 10 µm, bulk media, 1 kg	10 µm	100 Å	1 Each	Quantity
PLRP-S 100Å, 10 µm, bulk media, 1 kg. Fully porous PS/DVB reversed-phase particle support that is stable at elevated temperatures and a wide pH range(1-14). Available in wide range of particle and pore sizes for targeted applications Pressure Rating: 207 bar Particle Type: Fully Porous Hardware: Bulk Media Particle Size: 10 µm Pore Size: 100 Å See all product information
PL1412-2100	PLRP-S 100Å, 10 µm, bulk media, 10 g	10 µm	100 Å	1 Each	Quantity
PLRP-S 100Å, 10 µm, bulk media, 10 g. Fully porous PS/DVB reversed-phase particle support that is stable at elevated temperatures and a wide pH range(1-14). Available in wide range of particle and pore sizes for targeted applications Pressure Rating: 103 bar Particle Type: Fully Porous Hardware: Bulk Media Particle Size: 10 µm Pore Size: 100 Å See all product information
PL1412-4100	PLRP-S 100Å, 10 µm, bulk media, 100 g	10 µm	100 Å	1 Each	Quantity
PLRP-S 100Å, 10 µm, bulk media, 100 g. Fully porous PS/DVB reversed-phase particle support that is stable at elevated temperatures and a wide pH range(1-14). Available in wide range of particle and pore sizes for targeted applications Pressure Rating: 207 bar Particle Type: Fully Porous Hardware: Bulk Media Particle Size: 10 µm Pore Size: 100 Å See all product information
PL1812-6100	PLRP-S 100Å, 100 x 300 mm, 10 µm	10 µm	100 Å	1 Each	Quantity
PLRP-S 100Å, 100 x 300 mm, 10 µm. Reversed-phase HPLC column with fully porous PS/DVB particle support stable at elevated temperatures and a wide pH range(1-14). Available in range of particle and pore sizes for targeted applications Pressure Rating: 207 bar Particle Type: Fully Porous Hardware: SS Inner Diameter (ID): 100 mm Length: 300 mm Particle Size: 10 µm Pore Size: 100 Å See all product information
PL1812-6800	PLRP-S 100Å, 100 x 300 mm, 8 µm	8 µm	100 Å	1 Each	Quantity
PLRP-S 100Å, 100 x 300 mm, 8 µm. Reversed-phase HPLC column with fully porous PS/DVB particle support stable at elevated temperatures and a wide pH range(1-14). Available in range of particle and pore sizes for targeted applications Pressure Rating: 207 bar Particle Type: Fully Porous Hardware: SS Inner Diameter (ID): 100 mm Length: 300 mm Particle Size: 8 µm Pore Size: 100 Å See all product information
PL1912-3300	PLRP-S 100Å, 2.1 x 150 mm, 3 µm	3 µm	100 Å	1 Each	Quantity
PLRP-S 100Å, 2.1 x 150 mm, 3 µm. Reversed-phase HPLC column with fully porous PS/DVB particle support stable at elevated temperatures and a wide pH range(1-14). Available in range of particle and pore sizes for targeted applications Pressure Rating: 275 bar Particle Type: Fully Porous Hardware: SS Inner Diameter (ID): 2.1 mm Length: 150 mm Particle Size: 3 µm Pore Size: 100 Å See all product information
PL1912-3500	PLRP-S 100Å, 2.1 x 150 mm, 5 µm	5 µm	100 Å	1 Each	Quantity
PLRP-S 100Å, 2.1 x 150 mm, 5 µm. Reversed-phase HPLC column with fully porous PS/DVB particle support stable at elevated temperatures and a wide pH range(1-14). Available in range of particle and pore sizes for targeted applications Pressure Rating: 207 bar Particle Type: Fully Porous Hardware: SS Inner Diameter (ID): 2.1 mm Length: 150 mm Particle Size: 5 µm Pore Size: 100 Å See all product information
PL1912-3800	PLRP-S 100Å, 2.1 x 150 mm, 8 µm	8 µm	100 Å	1 Each	Quantity
PLRP-S 100Å, 2.1 x 150 mm, 8 µm. Reversed-phase HPLC column with fully porous PS/DVB particle support stable at elevated temperatures and a wide pH range(1-14). Available in range of particle and pore sizes for targeted applications Pressure Rating: 207 bar Hardware: SS Inner Diameter (ID): 2.1 mm Length: 150 mm Particle Size: 8 µm Pore Size: 100 Å See all product information
PL1912-5500	PLRP-S 100Å, 2.1 x 250 mm, 5 µm	5 µm	100 Å	1 Each	Quantity
PLRP-S 100Å, 2.1 x 250 mm, 5 µm. Reversed-phase HPLC column with fully porous PS/DVB particle support stable at elevated temperatures and a wide pH range(1-14). Available in range of particle and pore sizes for targeted applications Pressure Rating: 207 bar Particle Type: Fully Porous Hardware: SS Inner Diameter (ID): 2.1 mm Length: 250 mm Particle Size: 5 µm Pore Size: 100 Å See all product information

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Product Details

Features

Contain durable and resilient polymer particles that deliver reproducible results over longer lifetimes
Thermal and chemical stability for separations, optimization, sanitation, and regeneration increases selectivity and column lifetime
Comply with USP L21 designation
Particle sizes range from 3 μm to 50 μm
Pore sizes (100Å-4000Å) for separations of small molecules to large complexes, proteins, peptides, and polynucleotides

How It Works

Easily Desalt Proteins and Oligonucleotides

Both intact proteins and oligonucleotides may need desalting or buffer exchange to remove excess salt or interfering small molecules from the sample matrix. Excess salt can quickly foul the source of your mass spectrometer or reduce sensitivity. Matrix interferences can also diminish sensitivity or otherwise cause the sample to be incompatible with downstream analysis or use. AdvanceBio Spin columns offer simple and quick removal of small molecule matrix components from oligonucleotides longer than 10 nt or bp.

View Spin Columns

Understanding Ion Pair Reversed-Phase (IP-RP) Chromatography

Ion pair reversed-phase (IP-RP) is an analytical technique to retain and separate charged molecules not normally retained by a reversed-phase column. In the case of oligonucleotides, which are polar with an anionic backbone, the introduction of an alkylamine ion-pair agent facilitates an interaction with a C18 stationary phase. The positively charged nitrogen group creates an “ion pair” with the oligos’ anionic backbone. The alkyl chain of the amine is retained by the hydrophobic C18 stationary phase, creating retention and separation for the oligo.

View the PLRP-S Ordering Guide

Advantages of Polymeric Particles in Reversed-Phase

PLRP-S column particles are made of polystyrene-divinylbenzene (PS/DVB), an inherently hydrophobic polymer. The hydrophobicity and chemical stability of PS/DVB enables fine-tuning for selectivity of a broad range of analytes, including peptides, proteins, and oligonucleotides. PLRP-S is less prone to degradation compared to silica particles and has a wider pH range: 1-14. The absence of free silanols eliminates secondary interactions and enhances compatibility with formic acid mobile phase modifiers, often used in mass spectrometry for peptide and protein analysis.

Applications

Sensitive Intact Protein Quantitation of mAbs from Biological Matrix

This application note describes a method for the quantitative analysis of intact monoclonal antibodies (mAbs) in a serum matrix. HPLC method (PLRP-S column and higher column temperature) was optimized using conventional flow rate (0.5 mL/min) to achieve better reproducibility for high-throughput intact mAb analysis compared to the nanoflow protocol. The higher column temperature did not cause sample degradation; instead, it improved the MS sensitivity under such high flow conditions.

View the PLRP-S Column

Optimizing Analysis and Purification of Synthetic Peptides

Peptide therapeutics, including GLP-1 RAs, are often produced using solid-phase peptide synthesis. This multi-step process, where amino acids are sequentially added, can introduce process-related impurities. Achieving high purity and high yield at both analytical and preparative scales is essential for an efficient GLP-1 workflow. PLRP-S columns and bulk media are ideal for GLP-1 purification due to optimized pore sizes and easy scalability, making them well-suited for development and production environments.

Read the Application Note

Purification of Single Stranded RNA Oligonucleotides Using HPLC

This application note demonstrates the purification workflow of a single-stranded RNA Oligo by ion-pair reversed-phase high-performance liquid chromatography (HPLC) and how to scale up to prep using PLRP-S analytical and preparative column dimensions. HPLC is the preferred method for high-purity pharmaceutical production.

Videos

Purification Your Way: siRNA to mRNA

00:01:45

Tips to Optimize Lab Scale Oligonucleotide Purification - Part 1

00:10:49

Tips to Optimize Lab Scale Oligonucleotide Purification - Part 2

00:06:21

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Easily Desalt Proteins and Oligonucleotides