AdvanceBio Glycan Mapping
AdvanceBio Glycan Mapping columns leverage technology that optimizes results for MS and fluorescence detection. They can deliver glycan maps in less than 10 minutes - 40% less time than competing products - and can be ordered with a selection of standards for performance testing and retention mapping of labeled and unlabeled glycans.
- Glycan Analysis Columns
Product Details
- Speed: Obtain glycan maps in less than 10 minutes, superior results - in 40% less time than the competition
- High-resolution: Characterize both labeled and unlabeled glycans, and identify any glycosylation changes that occur due to process variables
- Flexibility: 2.7 µm superficially porous particles for high resolution with less backpressure. For UHPLC analysis 1.8 µm particles are an ideal choice
- Reproducibility: QA tested with a 2-AB labeled IgG N-linked glycan sample to ensure consistent quality and performance
- Application Notes
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Streamlined Workflows for N-Glycan Analysis of Biotherapeutics with LC/FLD/MS
Streamlined workflows for N-Glycan analysis of biotherapeutics using Agilent AdvanceBio Gly-X InstantPC and 2-AB Express sample preparation with LC/FLD/MS
- Application Notes
- English
- 23 Sep 2021
- 1.19 MB
Comparison of Relative Quantification of Monoclonal Antibody N-glycans Using Fluorescence and MS Detection
Comparison of Relative Quantification of Monoclonal Antibody N-glycans Using Fluorescence and MS Detection
- Application Notes
- English
- 11 Jul 2019
- 2.09 MB
A Comprehensive Approach for Monoclonal Antibody N-linked Glycan Analysis from Sample Preparation to Data Analysis
This application note demonstrates how to increase sample throughput for glycan characterization workflows using the Agilent biopharmaceutical solution.
- Application Notes
- English
- 23 Apr 2019
- 3.70 MB
Profiling Glycosylation of Monoclonal Antibodies at Three Levels Using the Agilent 6545XT AdvanceBio LC/Q-TOF
This application note demonstrates three approaches to quantitatively understand the glycosylation present for a given mAb.
- Application Notes
- English
- 23 Apr 2019
- 1.20 MB
Fast and Efficient HILIC Methods for Improved Analysis of Complex Glycan Structures
The Agilent 1.8 µm HILIC column reduced run time by 40% when separating N-linked glycans. The column also resolved complex antennary bovine fetuin glycans.
- Application Notes
- English
- 22 Nov 2017
- 1.38 MB
A Novel HILIC Column for High Speed N-linked Glycan Analysis
The Agilent HILIC-based AdvanceBio Glycan Mapping column is 40% faster, with high resolution, efficiency, and chemical stability for at least 5,000 column volumes.
- Application Notes
- English
- 22 Nov 2017
- 419.57 KB
Sensitive and Reproducible Glycan Analysis of Human Immunoglobulin G
This Application Note demonstrates the sensitive and reproducible analysis of N-linked glycans of human immunoglobulin G with a novel HILIC column and fluorescence detection.
- Application Notes
- English
- 22 Nov 2017
- 840.43 KB
Separation of IgG Glycopeptides using HILIC-LC/MS in Comparison to RP-LC/MS
An Agilent 2.7 µm HILIC column revealed a glycopeptide not seen by RP. HILIC can be orthogonal and complementary to RP for better retention of hydrophilic peptides.
- Application Notes
- English
- 22 Nov 2017
- 1.80 MB
Analysis of Monoclonal Antibody N-glycans by Fluorescence Detection and Mass Selective Detection Using LC/MSD XT
This app note demonstrates the performance of an Agilent LC/MSD to solve ambiguous peak assignments for the analysis of N-glycosylation of therapeutic proteins
- Application Notes
- English
- 14 Jan 2020
- 775.86 KB
- Brochures
- Flyers
- User Manuals
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Column User Guide for Agilent AdvanceBio Glycan Mapping Columns
Column User Guide for Agilent AdvanceBio Glycan Mapping Columns. Application-specific columns designed for the characterization of protein glycosylation.
- User Manuals
- English
- 10 May 2019
- 419.53 KB