Amide HILIC & Glycan Mapping HPLC Columns

Features

Speed: Obtain glycan profiles in less than 10 minutes, superior results - in 40% less time than the competition
High-resolution: Characterize both labeled and unlabeled glycans, and identify any glycosylation changes that occur due to process variables
Reproducibility: QA tested with 2-AB- or InstantPC-labeled N-linked glycan samples to ensure consistent quality and performance
Robustness: Amide HILIC columns provide excellent long-term stability, even at high temperatures (up to 80C)

Tools

Bio HPLC Columns eSelector Tools

Intuitive process to help you select the right chromatography column for your biopharmaceutical applications.

How It Works

Amide HILIC Retention Time Prediction Tool for InstantPC and 2-AB Glycans

Agilent now offers a tool which uses data from calibration standards to predict retention times for many N-glycans present in biotherapeutic molecules. The tool is free to use and works with both InstantPC and 2-AB labels, used in conjunction with the AdvanceBio Amide HILIC 2.1 x 150, 1.8 µm LC column.

Predicts retention times for over 100 glycans
Requires only calibration data (labeled dextran or maltodextrin ladder, plus optional individual refinement standards) so no sample data needs to be shared
Works with the AdvanceBio Amide HILIC 2.1 x 150, 1.8 µm LC column

Access the Free Tool

Learn More

Greater Accuracy and Efficiency for Complex Biopharmaceutical Separations

Before the chromatographic analysis, you must cleave and isolate the N-linked glycans from the reaction mix, then derivatize with a fluorescent label before final cleanup. N-Glycan sample preparation kits provide all reagents needed to prepare samples.

One part number to order the full sample-prep workflow.
User guide with tips and tricks for preparing complex samples.
Kits for each step of the workflow have separate part numbers for flexibility.
Kits for 24 and 96 samples to avoid wasting expensive reagents.

Simplify the Biopharma HPLC Columns Search with Intuitive eSelector Tools

Use the Biopharma HPLC Column eSelector Tools to quickly and easily find the right Biopharma HPLC columns. Begin selecting your column by one of the following:

Your unique application area
Your separation mode
Another vendor's part number

Use the eSelector Tool

Applications

Streamlined Workflows for N-Glycan Analysis of Biotherapeutics

The characterization of N-glycans is essential for the biotherapeutic development process. This application note describes the preparation and analysis of released N-glycans from biotherapeutic glycoproteins using two labels.

Literature

Key Literature

Complete N-Glycan Workflow Solutions

Agilent offers tools and support for every step of your glycan sample preparation and analysis workflow.

Flyers
English
09 Oct 2024
387.09 KB
PDF

Glycan Peak Assignment Tool for Improved Accuracy in N-Glycan HILIC/FLD Analysis

Agilent Glycan Peak Assignment Tool for improved accuracy in N-glycan analysis with separations using the AdvanceBio Amide HILIC column.

Application Notes
English
30 May 2024
573.41 KB
PDF

Improved HILIC for LC/FLD/MS Analysis of Released N-Glycans

In this application note, we profiled the N-glycans of several monoclonal antibodies with the new Agilent AdvanceBio Amide HILIC column.

Application Notes
English
15 Nov 2023
979.71 KB
PDF

Application Notes

Brochures

Catalogs

Support

FAQs

Glycan Peak Assignment Tool Frequently Asked Questions (FAQs)

Bio LC Column User Guides

User guides contain helpful information to ensure the best performance from your column, such as operating parameters, cleaning and storage guidelines, usage tips, and suggested starting conditions.

User Manuals

AdvanceBio Gly-X Technology

Gly-X sample prep shortens time to N-glycan analysis with fast, in-solution workflows

Category: N-Glycan Sample Preparation & Analysis

AdvanceBio InstantPC Labeled Glycans

For qualitative standards in LC/FLD and LC/MS, InstantPC labeled glycans produce reliable results

Category: N-Glycan Sample Preparation & Analysis

AdvanceBio 2-AB Labeled Glycan Standards and Libraries

Standards and libraries provide high confidence in identification of samples

Category: N-Glycan Sample Preparation & Analysis

AdvanceBio Spin Columns

Efficient sample preparation of proteins or oligonucleotides under aqueous conditions in 10 to 15 minutes.

Category: Biomolecule Sample Preparation

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News

Agilent Launches New Tool To Identify InstantPC- or 2-AB-labeled N-glycan Peaks

The free Glycan Peak Assignment Tool was developed to provide additional confidence in identifying InstantPC- or 2-AB-labeled N-glycan peaks by predicting retention times of over 100 glycans. The new tool does not require users to upload sensitive data from N-glycan samples.

01 May 2024

Agilent Launches New AdvanceBio Spin Columns For Desalting and Buffer Exchange

AdvanceBio Spin Columns address the need for efficient sample preparation of proteins or oligonucleotides under aqueous conditions, preserving native structure with suitable ionic strength and pH. Users can easily and quickly remove excess salts or undesired small molecules from their sample matrix or exchange the sample matrix between different buffer conditions. These gel-filtration-based spin columns are available in analytical and semipreparative scale columns for single samples, as well as 96-sample plates for high-throughput settings.

01 Apr 2024

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AdvanceBio Amide HILIC & Glycan Mapping Columns

Product Details

Bio HPLC Columns eSelector Tools

Amide HILIC Retention Time Prediction Tool for InstantPC and 2-AB Glycans

Greater Accuracy and Efficiency for Complex Biopharmaceutical Separations

Simplify the Biopharma HPLC Columns Search with Intuitive eSelector Tools

Streamlined Workflows for N-Glycan Analysis of Biotherapeutics

Complete N-Glycan Workflow Solutions

Glycan Peak Assignment Tool for Improved Accuracy in N-Glycan HILIC/FLD Analysis

Improved HILIC for LC/FLD/MS Analysis of Released N-Glycans

Glycan Peak Assignment Tool Frequently Asked Questions (FAQs)

Bio LC Column User Guides

AdvanceBio Gly-X Technology

AdvanceBio InstantPC Labeled Glycans

AdvanceBio 2-AB Labeled Glycan Standards and Libraries

AdvanceBio Spin Columns

Agilent Launches New Tool To Identify InstantPC- or 2-AB-labeled N-glycan Peaks

Agilent Launches New AdvanceBio Spin Columns For Desalting and Buffer Exchange

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