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内切糖苷酶

内切糖苷酶

安捷伦内切糖苷酶可将寡糖从糖蛋白及其他底物中释放出来。这些酶可用于在分析前对糖蛋白进行去糖基化,或用于释放 N-糖以进行标记和分析。 针对 N-糖分析,安捷伦提供了一种用于释放完整 N-糖的天冬酰胺酰胺酶 — PNGase F。PNGase F 可在 N-糖最内侧的 GlcNac 与 N-糖所连接的天冬酰胺 (Asn) 残基之间进行切割

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