Comparing LC-UV and LC-MS Methods for Spent Media Analysis
Spent media analysis helps monitor cell health and nutrient and metabolite levels to quickly identify and troubleshoot any problems. Assays are traditionally done individually for metabolites such as glucose, lactate, and amino acids. LC-UV with sample derivatization has been the primary method for amino acid analysis, but sample derivatization can be a bottleneck. Alternative technologies like LC-MS enables an increase in the number and types of metabolites that can be combined into a single assay.