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Fermentation-derived Proteins Testing

Fermentation has been used for centuries to produce, preserve, and improve the nutritional value of foods and beverages. As new technologies enhance our ability to exploit this process, microbial-based protein production is emerging as a powerful, flexible approach for producing proteins for food use. However, challenges unique to this approach remain, including issues of target selection, bioprocess optimization, and product formulation. Agilent's GC, LC, GC/MS, LC/MS, and ICP-MS analytical solutions help food scientists tap the full potential of fermentation-based protein production.

If you have questions about the analysis of fermentation- and microbe-based proteins or would like to be contacted by an Agilent representative, let us know below.

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Comparing LC-UV and LC-MS Methods for Spent Media Analysis

Spent media analysis helps monitor cell health and nutrient and metabolite levels to quickly identify and troubleshoot any problems. Assays are traditionally done individually for metabolites such as glucose, lactate, and amino acids. LC-UV with sample derivatization has been the primary method for amino acid analysis, but sample derivatization can be a bottleneck. Alternative technologies like LC-MS enables an increase in the number and types of metabolites that can be combined into a single assay.


Amino Acid Analysis in Fermentation Media

This application note highlights a streamlined workflow for the analysis of underivatized amino acids for bioreactor cell culture monitoring by HILIC-LC/Q-TOF MS. The pH stability of the Agilent AdvanceBio MS Spent Media LC column allowed for cell media, feedstock, and cell waste products to be monitored simultaneously.



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