Exploring the Role of Metabolism in Immune Function
Whether the research focus is on a specific immune cell type, signaling pathway, or substrate usage, immunologists are actively exploring the mechanisms that drive and perpetuate antigen recognition and response. Immunometabolism has emerged as a crucial component to understand the connection between metabolic pathways and immune responses. Agilent Cell Analysis technology is at the forefront, providing powerful and effective tools to explore this burgeoning field of research.
Tuberculosis (TB) is the top infectious killer in the world with a major cause of these deaths being antimycobacterial drug resistance. Hence, host-directed therapies are being increasingly considered as adjunctive treatment for TB. This requires a more comprehensive knowledge of how Mycobacterium tuberculosis (Mtb), the causative agent of TB, modulates the metabolism of the host to subvert the host’s immune response. Using the Seahorse XF Technology, we investigated how Mtb reprogrammed the bioenergetic metabolism of the human host in vitro and ex vivo.
This tutorial describes a novel assay that measures immune cell activation from within minutes of activation throughout the fully developed activated phenotype in both a qualitative and quantitative manner. This provides a sensitive window of opportunity to identify and characterize attributes that can drive immune responses to desired outcomes.
Join us for our eSeminar presented by Dr. Ping-Chih Ho to learn about Agilent Cell Analysis applications in cancer immunotherapy research.
The aberrant accumulation of regulatory T cells (Tregs) in tumors promotes immunosuppressive features of the tumor microenvironment (TME). Although depleting Tregs unleashes antitumor immunity and reprograms the immune status of the TME, the therapeutic potential of Treg-targeting is largely compromised due to autoimmunity induced by systemic loss of Tregs. Given that Tregs reside in different locations where they contain distinct nutrient composition, metabolic adaptation should be engaged by Tregs to support their tissue retention and tissue-dependent behavior. This talk will discuss how we can selectively demolish intramural Tregs by targeting unique metabolic preference of intratumoral Tregs and how this approach can be utilized for cancer research.