Exploring the Role of Metabolism in Immune Function
Whether the research focus is on a specific immune cell type, signaling pathway, or substrate usage, immunologists are actively exploring the mechanisms that drive and perpetuate antigen recognition and response. Immunometabolism has emerged as a crucial component to understand the connection between metabolic pathways and immune responses. Agilent Cell Analysis technology is at the forefront, providing powerful and effective tools to explore this burgeoning field of research.
Tuberculosis (TB) is the top infectious killer in the world with a major cause of these deaths being antimycobacterial drug resistance. Hence, host-directed therapies are being increasingly considered as adjunctive treatment for TB. This requires a more comprehensive knowledge of how Mycobacterium tuberculosis (Mtb), the causative agent of TB, modulates the metabolism of the host to subvert the host’s immune response. Using the Seahorse XF Technology, we investigated how Mtb reprogrammed the bioenergetic metabolism of the human host in vitro and ex vivo.
This tutorial describes a novel assay that measures immune cell activation from within minutes of activation throughout the fully developed activated phenotype in both a qualitative and quantitative manner. This provides a sensitive window of opportunity to identify and characterize attributes that can drive immune responses to desired outcomes.
An emerging theme in immunology is that metabolic reprogramming and lymphocyte function are intricately linked. However, why T cells adopt specific metabolic programs, and the impact that these programs have on T cell function and, ultimately, immunological outcomes remain unclear. Using the Seahorse XF Extracellular Flux Analyzer, we discovered that T cells regulate their metabolism in response to both immune stimuli and environmental cues.