TCID50 (50% tissue culture infectious dose) is an endpoint dilution assay used to measure infectious viral titer. This method involves infecting cultured cells within a microplate and is useful for viruses that do not form plaques. After an incubation period, microplate wells are classified as infected or not infected. The viral dilution at which 50% of the wells are infected is used to calculate the TCID50. Determination of viral infection can be accomplished with automated fluorescence imaging and either fluorescently tagged viral particles or fluorescent cell viability markers. A label-free cytopathic effect (CPE) assay can also be employed to determine TCID50.
A rapid viral infectivity assay using AVIATM and the Agilent BioTek Cytation cell imaging multimode reader dramatically accelerates results, reduces costs, and increases precision.
This application note demonstrates the ability of the Agilent BioTek Cytation 5 cell imaging multimode reader and Agilent BioTek Gen5 microplate reader and imager software to determine infection levels of human immunodeficiency virus (HIV) with HeLa cells in culture.
This poster demonstrates the use of label-free, impedance-based xCELLigence RTCA technology for virus and antibody titer determination as well as for antiviral drug screening.
The BioSpa Live Cell Analysis System enables multiplate label-free TCID50 analysis when integrated with the Cytation Live Cell Imager.
In this featured article, the authors provide a detailed description of a robust, automated method for conducting TCID50 assays to determine viral titers with the Cytation 5 Cell Imaging Multimode Reader. This label-free imaging approach for determining cell confluence was used to identify CPE-positive and CPE-negative wells and to assess virus-based therapeutics. Additionally, the Agilent Bravo automated liquid handling platform is incorporated into the assay setup to increase efficiency and walk-away time.
Agilent BioTek automated imaging systems and liquid handlers provide optimized solutions for a broad range of virology applications.
