Rapid, Image-Based Viability and Outgrowth Analysis for Neurotoxicity Assays
Single-step labeling and high-throughput, automated live-cell analysis to capture complex and specific neuron responses across a panel of neurotoxic treatments.
Stem Cell Biology
Unlock the Full Potential of Your Stem Cells
Stem cell research provides mechanistic insights into disease, aids regenerative medicine development, and offers an alternative approach to test the liability and effectiveness of new drugs. Mesenchymal stem cells (MSCs) can differentiate ex vivo or in vivo into adipocytes, chondrocytes, and osteocytes, which can be used for tissue repair or disease modeling. They can also suppress immune rejections and inflammatory responses when used alone or with other treatments.
Advancements in this field have been impaired by a lack of cell‑to‑cell consistency and quality control (QC) standards, leading to failed or misinterpreted results. Additionally, the reprogramming of somatic cells, such as fibroblasts, to a pluripotent state has a dramatic effect on the metabolic requirements of the cell, inducing a cancer-like (Warburg) metabolic shift from oxidative phosphorylation to glycolysis. There is increasing evidence illustrating that the metabolic shift in cells, such as induced pluripotent stem cells (iPSCs), has a significant importance in establishing pluripotent identity, with metabolic phenotype being a valuable indicator of pluripotency that can be used to improve differentiation efficiency and protocol optimization.
Explore Agilent cell analysis technologies to gain deeper insight into stem cell function and uncover mechanisms involved in cellular reprogramming and differentiation.
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Stem Cell Applications
Accurately Assess the Function and Quality of Stem Cell-Based Products
Predict functional capacity and assess variability of mesenchymal stem cells (MSCs) more effectively with Agilent xCELLigence RTCA. Continuously measure integrated changes in cell number, attachment, and morphology to ensure consistency between passages.
Authenticate Disease Models and Improve Protocol Efficiency Using Metabolic Measurements
Validate the loss and gain of pluripotency and lineage commitment in stem cell disease models using real-time metabolic measurements to gain confidence in your disease models and improve protocol efficiency.
Long-Term Electrical Pacing Improves Maturation of hiPSC-CMs
Human-induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs) are currently being used for drug discovery, toxicology, and cardiac disease research. Improve the maturation status of hiPSC-CMs in a consistent and scalable manner with this high-throughput workflow.
Immunophenotyping by Flow Cytometry
Multi-parameter flow cytometry is a valuable tool for validation of human pluripotent stem cells (hPSCs) to ensure the quality and differentiation status of cells. Complex multicolor immunophenotyping experiments are simple to perform with Agilent NovoCyte Flow Cytometers.
Explore Next Generation Solutions for Cell Therapies
In this virtual lab, explore stem cell solutions for quantitative assessment of predictive biomarkers, bioenergetics, real-time cell growth and proliferation, extracellular vesicle detection, and evaluation of cytokine profiles. Cellular therapies using stem cells are increasingly being utilized in various clinical trials for their immunomodulatory behavior as well as for regenerative medicine applications. Discover new solutions for optimizing stem cell maintenance, growth and differentiation conditions to ensure high quality cells for therapeutic purposes.
Quality Control and Functional Assessment of Mesenchymal Stem Cells for Cellular Therapies
Assess bone marrow-derived mesenchymal stem cells (BMSCs) using Agilent xCELLigence RTCA by continuously monitoring cellular status under physiologically relevant conditions. Multiple quantitative parameters can be derived from the data to characterize BMSCs and estimate cell potency and function. Read the application note to learn how to effectively estimate BMSC functional capacity (i.e., differentiation potential) for preclinical quality control assessment.
Agilent Seahorse XF Live-Cell Metabolism Solutions for Stem Cell Research
Stem cells, somatic cells, and differentiated cells exhibit specific and distinct metabolic signatures. Measurable metabolic switching events occur at multiple stages when cells become reprogrammed; enter and exit a pluripotent state; begin to differentiate; and terminally differentiate. Download our brochure, Agilent Seahorse XF Live-Cell Metabolism Solutions for Stem Cell Research, to learn how functional real-time metabolic measurements can provide insights into stem cell processes for disease model optimization and validation.
Assessment of Cardiomyocyte Disease Models
The real-time multiplexed evaluation of the functional activity of beating cardiomyocytes using the Agilent xCELLigence RTCA CardioECR system allows for depicting and recapitulating disease phenotype of cardiomyocytes differentiated from human-specific induced pluripotent stem cell (PS-iPSC). Read the application note to learn how to investigate their pharmacological responses, which could potentially provide information about underlying disease mechanisms.