Real-Time Cytotoxicity Assays

Monitor and quantify growth (either label or label-free) of 3D single tumor spheroids with Agilent xCELLigence RTCA eSight—all inside your incubator. Perform simple and effective detection of 3D tumor cell growth and shrinkage via live cell imaging utilizing mix and read reagents to fully gauge spheroid proliferation and cytotoxicity. The simple 3D cellular viability or toxicity readouts generate reproducible data, resulting in deeper and more meaningful biological insights into your drug discovery assays.

Tumor spheroid growth is traditionally examined visually by standard microscopy; however, this can be time consuming and labor intensive. The xCELLigence RTCA eSight system provides an image-based high-throughput screening method for 3D tumor spheroids, offering a more automated approach with less hands-on time and simple quantification of tumor growth or shrinkage. With this easy workflow, simply add cell treatments and/or reagents (eLenti, eTox, eAnnexin, or eCapase) at day 3 for quantitative analysis.

Easily perform high-throughput compound screening and profiling with the xCELLigence RTCA HT instrument, which provides real-time monitoring of changes in cell attachment, morphology, and growth characteristics as a response to cytotoxic agents. Make informed decisions about the timing of cell treatments and easily generate dose-response curves at multiple time points, without increasing the workload.

See how Alberta Centre for Toxicology scientists developed a label-free, real-time cellular assay for source water monitoring. The scientists collected a total of 436 river water samples for assessment of water cytotoxicity. The assay used HepG2 (human hepatocarcinoma) cells and cytotoxicity response was tested at dilutions of 80%, 60%, 40%, 30%, 20%, and 10% of the original water samples. The Agilent xCELLigence RTCA system monitored HepG2 cell growth following exposure to the combined toxic effect present in water samples.