Cancer cytogenetics research requires a comprehensive sample quality control (QC) and data interpretation workflow to enable sensitive and accurate genome-wide analysis.
Agilent’s microarray platform delivers advanced analysis of CNCs and cnLOH for a wide variety of sample types. Our system employs a highly accurate buffer solution and optimized labeling technique for robust aberration detection. Agilent CGH microarrays are the standard of excellence for characterization of aberrations, with over 15 years of research use and 14,000 published papers.
It’s easier, faster, and more cost effective to achieve your research goals with Agilent’s microarray solution. From sample quality control to data interpretation, Agilent’s broad portfolio of CGH products for cancer cytogenetics help you drive lab efficiency and achieve accurate results.
_0000_Cancer Cytogenetics.jpg)
_0001_Featured Video.jpg)
Easily create cancer-focused catalog CGH and custom microarray designs using SureDesign, a web-based application that harnesses the adaptability of SurePrint oligo manufacturing technology.
Agilent's online SureDesign CGH design wizard makes custom array design intuitive and fast. Create, cross validate, and collaborate for optimal research results.
Agilent CGH+SNP microarrays demonstrate superior copy number calling for cytogenetic profiling of hematological cancer samples.
_0002_Featured Literature.jpg)
The ability to detect copy number changes in single exons in clinical research samples may be important in identifying aberrations that regulate disease susceptibility and risk.
Exon-level deletions and duplications may not be detected by NGS due to size, and therefore complimentary approaches are needed.1
1Reference: Eur J Hum Genet. 2014 Jan;22(1):79-87
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3865406/
Yes, very small copy number changes in regions associated with disease can be identified. For example, both Agilent’s GenetiSure Cancer CGH+SNP Research 2x400K microarray and GenetiSure Postnatal CGH+SNP Research 2x400K microarray contain nearly 300,000 CGH probes, allowing for the detection of small aberrations in regions that are significant for cancer studies.
Low level mosaicism, as low as 8%2, can be picked up, eliminating the need to enrich for tumor cells. Clonal fraction estimation is reported out in Agilent's CytoGenomics Software, providing value add to the analysis.
2 Agilent CGH+SNP Microarrays Demonstrate Superior Copy Number Calling for Cytogenetic Profiling of Hematological Cancer Samples (https://www.agilent.com/cs/library/applications/5991-0409EN.pdf) Valli et al. Molecular Cytogenetics 2011, 4·13)
LOH of 5-10 Mb is suggested by ACMG guidelines for significance. However, if you want to future-proof your data, Agilent arrays have improved resolution down to 2.5 Mb. Some array manufacturers have a lower theoretical LOH resolution, however, it is unclear if this provides any meaningful benefit and may increase the chance of false positives in your data. In addition, Agilent’s true copy number and SNP probe platform can confidently call copy number and copy-neutral events, providing advantages in data quality over SNP-centric platforms.
Designing in SureDesign, Agilent's free online design application, has never been easier. There are many benefits in designing your own microarrays and Agilent is leader in customization.
Targeted microarrays enable cancer researchers to focus on genomic regions that are associated with disease. A combination of targeted probe content for selected genomic regions at high resolution and genome-wide probe coverage at lower resolution is the ideal microarray design choice for most applications. This approach has been supported by key consortia such as the Cancer Genomics Consortium (CGC/CCMC).
The flexibility of Agilent's microarray customizable probe content offers real-time customization to meet your exact needs.
This key feature offers laboratories the ability to respond to new genetic discoveries and associations as they are being made on a daily basis, and these new findings may suggest opportunities to modify probe content as the data becomes available.
Agilent’s customization capabilities are backed by over 15 years of quality, flexibility and experience.
Our SurePrint oligo synthesis technology is a proven platform that is flexible to accommodate your unique requirements and delivers catalog-quality probes for custom designs. Tap into SureDesign and select from a database of 28 million probes to input target genes of interest and create a design that fits your needs. Custom arrays do not cost more than arrays with pre-designed content and, whether ordering 1 or 100s of custom arrays, our platform delivers flexibility to meet your growing needs.
Ensure that aberrations are not missed when targeting cancer regions of the genome from the COSMIC and CGC databases with the GenetiSure microarray
_0004_Microarray Reagents.jpg)
Deliver efficient results with our CMA kits and reagents including DNA purification columns, Human Cot DNA and Hybridization Kits, and Labeling kits.
_0003_Microarray Buffers.jpg)
Obtain optimal performance for CGH experiments using Agilent arrays with our high-quality reagents and buffers for microarray processing.
Achieve outstanding sensitivity and resolution when analyzing genomics and cytogenetics microarrays. Agilent’s SureScan platform maximizes lab space without compromising on results; improve your analysis sensitivity and maintain the broadest dynamic range.
_0001_SureDesign Software.jpg)
Create custom CGH microarrays from a database of >28 million highly curated probes with our SureDesign software. Customize content and format to your needs. Your Agilent microarray can be manufactured to order, using the same robust process as our catalog products.
_0006_Microarray Software.jpg)
Streamline your workflow for processing, analyzing, and reporting of cytogenetic measurements with Cytogenomics research software. This includes CNCs and cnLOH generated with a diverse range of human sample types on Agilent CGH-only and CGH+SNP microarrays.
_0000_Alissa Informatics.jpg)
Enable automated variant assessment for structural variants including CNVs, LOH, and translocations with the Alissa Clinical Informatics Platform. Optimize workflows across technologies and applications for greater efficiency and shorter time to results.
For Research Use Only. Not for use in diagnostic procedures.