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Be Agilent Sure in Intact Protein Analysis

Be Agilent Sure When Characterizing Intact Proteins

You can count on Agilent innovations to help you sensitively and confidently characterize intact proteins throughout your drug discovery and development pipeline.

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Monoclonal antibody (mAb) drugs are challenging to analyze due to their high molecular weight. Intact protein analysis lets you rapidly measure the molecular weight of a mAb product. It also enables you to determine mAb heterogeneity—such as post-translational modifications (PTMs), antibody drug conjugates (ADCs), sequence variations, or degradation products.

Click each icon to learn how Agilent can help at every step of your intact protein analysis workflow.







The Agilent AssayMAP Bravo liquid handling system automates complex sample preparation. Its robotic precision improves reproducibility across users and labs. What’s more, it allows users to scale experiments up and down from one or two samples to tens or hundreds. Other advantages include:

  • The ability to process 8–96 samples in parallel
  • Precise flow control across the cartridge bed
  • Quantitative binding and elution
  • High-efficiency washing
  • Small elution volumes

The Agilent 1290 Infinity II LC system sets the benchmark in UHPLC to deliver maximum efficiency.

Agilent PLRP-S columns are inherently hydrophobic, and provide a reproducible material that is free from silanols and heavy metal ions. Their macroporous polymer particles deliver HPLC separations over the widest pH range.

The Agilent 6545XT AdvanceBio LC/Q-TOF system gives you best-in-class data quality and analytical sensitivity for intact protein analysis.

Advanced data mining and processing tools in the Agilent MassHunter software suite help you quickly and accurately extract all available information from your samples. What’s more, BioConfirm software can identify and confirm variants before you start expensive testing.

MassHunter WalkUp transforms mass spectrometry from a limited-access service into a tool that anyone can use.

The Agilent OpenLab Chromatography Data System with MatchCompare software removes the subjectivity of human evaluation/comparison while making data analysis less complex and time consuming.

Agilent CrossLab supports Agilent and select non-Agilent instruments. Our experts provide consultative support for workflow enablement, lab analytics, compliance, inventory management, and asset management, including relocation services.

Everything you need to streamline your intact protein analysis


Looking for detailed information on new techniques or technologies? Our wide range of application notes, videos, on-demand webinars, and other resources can help you achieve more accurate, reproducible results.

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On-demand webinars

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Intact Protein Analysis in a Multi-User/Multi-Project Walk-Up Environment

Explore a high resolution/high mass accuracy walk-up solution—including hardware and software—for analyzing intact proteins.

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Tackling Protein Characterization Challenges with LC/Q-TOF: From Sample Prep to Results

Learn how the latest biopharma innovations can give you confidence in the face of ever-changing analytical characterization challenges.

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Need to reproducibly measure large biomolecules up to 30,000 m/z?

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Precise Characterization of Intact Monoclonal Antibodies by the Agilent 6545XT AdvanceBio LC/Q-TOF

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From Sample Prep to Data Analysis: Agilent Biopharma Workflow Video

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MassHunter BioConfirm DAR Calculator: Accelerate ADC Research Video

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Designed for multiple biopharma workflows

The Agilent 6545XT AdvanceBio LC/Q-TOF system

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Agilent AdvanceBio columns

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