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Be Agilent Sure in Glycans

When Profiling Intact Glycoforms And Released Glycans, You Need To Be Agilent Sure

You can count on innovative Agilent techniques and technologies to help you perform faster glycan analysis without sacrificing data quality or spectral resolution.

Many of today’s blockbuster drugs are proteins called monoclonal antibodies (mAbs). Because glycans present on protein surfaces can affect drug functionality, you must carefully analyze the glycan profile of every protein batch during every manufacturing stage.

You can perform this analysis using two analytical methods:

  • Monitoring glycoforms at the intact and subunit protein level allows fast, efficient screening for major glycan content
  • Releasing N-glycans, followed by fluorescence labeling, gives the best quantitation, and allows detailed glycan structure identification by mass spectrometry

Click each icon to learn how to be Agilent sure at every step of your glycans workflow.

Sample prep






The Agilent AssayMAP Bravo liquid handling system automates complex sample preparation for glycan analysis by integrating with Prozyme GlykoPrep® kits. Its robotic precision improves reproducibility across users and labs. What’s more, it allows users to scale experiments up and down from one or two samples to tens or hundreds. Other advantages include:

  • The ability to process 8–96 samples in parallel
  • Precise flow control across the cartridge bed
  • Quantitative binding and elution
  • High-efficiency washing
  • Small elution volumes

Achieve superior outcomes with the high-performance Agilent 1290 Infinity II LC system, and increase peak capacity for challenging separations with 2D-LC.

Intact glycoforms: Agilent PLRP-S 1000 A columns contain polymer particles that deliver reproducible results over longer lifetimes.

Released glycans: Agilent AdvanceBio Glycan Mapping columns deliver FAST, high-resolution, reproducible glycan identification using HILIC.

The AdvanceBio 6545XT LC/Q-TOF pushes your protein analysis to new levels of performance and reproducibility. Its autotune feature allows you to optimize the system for large proteins or small labile structures, such as glycans.

Advanced data mining and processing tools in the Agilent MassHunter Software suite help you quickly and accurately extract all available information from your samples. What’s more, BioConfirm software can identify and confirm PTMs before you start expensive testing.

Agilent OpenLab Chromatography Data System with MatchCompare software removes the subjectivity of human evaluation/comparison while making data analysis less complex and time consuming.

Agilent CrossLab service plans care for your entire laboratory with rapid onsite support, multivendor instrument service, remote instrument diagnostics, and more. From maintenance and repairs… to compliance… to relocation… our expert engineers provide insights that can improve efficiency for one instrument, or an entire lab.

Everything you need to streamline glycans analysis

Looking for detailed information on new techniques or technologies? Our wide range of application notes, videos, on-demand webinars, and other resources can help you achieve more accurate, reproducible results.

Learn more

Featured webinar

Automating Mass Spectrometry Based Glycan Identification from Sample Prep to Data Analysis

Fast, high-resolution and reproducible glycan identification is now possible. Learn more about how mAb glycosylation can be monitored at the intact, subunit, and released glycan levels in this essential webinar led by expert scientists.

Watch now

Featured resources for confident glycans analysis

Application notes

Intact glycoforms: Profiling Glycosylation of Monoclonal Antibodies at Three Levels Using the Agilent 6545XT AdvanceBio LC/Q-TOF Download now

Released glycans: A Comprehensive Approach for Monoclonal Antibody N-linked Glycan Analysis from Sample Preparation to Data Analysis Download now

On-demand webinar

Three Steps to Glycan Success
Learn how a single set of robust and easy-to-adopt tools can tackle multiple levels of protein characterization Watch now

Make your biopharma workflow more productive

Designed for multiple biopharma workflows: Agilent 6545XT AdvanceBio LC/Q-TOF system

Look closer

Analyze highly complex biotherapeutic molecules: Agilent AdvanceBio columns

Measure what matters

Produce answers… not just data: Small molecule analysis

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