Skip to main content

Be Agilent Sure of Your Biologic's Critical Quality Attributes

The importance of understanding the attributes of a biologic drug, and the processes used to create it, cannot be underestimated.

Hero Image

Measure what matters

Agilent AdvanceBio columns are designed and produced to provide results you can be sure of when analyzing highly complex biotherapeutic molecules and monitoring their purity, potency, and other critical quality attributes.


Which Critical Quality Attribute are you monitoring?

Amino Acid and Cell Culture Analysis

Is our cell culture media optimal for mAb production? Agilent AdvanceBio cell culture workflows cover all your needs.

View products

Amino Acid & Cell Culture Analysis

Composition of cell culture media is critical to the quantity and quality of the biotherapeutic produced.

LC/UV with sample derivatization is a simple and robust approach to amino acid analysis.

An LC/MS-based workflow allows you to bypass sample derivatization and simultaneously detect other cell culture metabolites.

View products

Intact & Subunit Analysis Using Reversed Phase

Are there impurities that we aren’t resolving? Agilent wide pore columns come in a range of chemistries to give you the resolution, selectivity, and performance needed.

View Products

Intact & Subunit Analysis Using Reversed Phase

Biotherapeutic proteins are often heterogeneous, containing a mixture of fragments and other product-related impurities.

AdvanceBio RP mAb was specifically designed with the optimum pore size for monoclonal antibodies.

The polymer-based chemistry of PLRP-S gives excellent peak shape under formic acid conditions for MS detection.

View all column options

Intact Analysis Using Hydrophobic Interaction

Does the native molecule have product related impurities that could impact stability or potency? Agilent AdvanceBio HIC columns will resolve various protein variants (PTMs) including oxidation in mAbs and drug-antibody species observed in ADC’s.

View Products

Intact Analysis Using Hydrophobic Interaction

AdvanceBio HIC columns are designed to quickly determine post translational modifications without compromising performance.

A single chemistry is suitable for multiple applications. The chemistry is optimized to provide increased resolution and peak shape with enhanced selectivity to measure oxidation and drug to antibody ratios.

View Products

Aggregate and Fragment Analysis

Is our aggregation measurement accurate? Agilent AdvanceBio SEC columns deliver accurate, precise quantitation for mAb aggregation and protein analysis.

View Products

Aggregate & Fragment Analysis

Aggregation may trigger undesirable immunogenic response, making it imperative to accurately measure aggregation levels.

The inert chemistry of AdvanceBio SEC column gives excellent peak shape without mobile phase additives, ensuring the integrity of your measurement.

View Products

Charge Variant Analysis

Is purification or formulation leading to protein oxidation? Agilent Bio IEX and Bio mAb columns separate the charge variants caused by a wide variety of PTMs.

View products

Charge Variant Analysis

Charge variants, especially in the complementarity determining region of mAbs, can significantly impact efficacy.

Agilent Bio mAb columns are specially designed for separation of monoclonal antibody charge variants.

Agilent Bio IEX is available in four chemistries to meet any ion exchange need.

View all column options

Peptide Mapping

Can we detect the PTMs that matter most? Agilent AdvanceBio columns offer complementary column choices to solve all your peptide-mapping challenges.

View products

Peptide Mapping

Peptide mapping is used to confirm primary sequence, as well as identify and quantify critical PTMs.

The AdvanceBio Peptide Mapping column offers robust performance for a wide range of proteins.

AdvanceBio Peptide Plus offers ideal peak shape under formic acid conditions for MS detection.

View products

Glycan Analysis

Do we have a complete picture of our protein’s glycosylation? Agilent AdvanceBio Glycan Mapping columns are part of a complete workflow solution.

View products

Glycan Analysis

As glycans are instrumental in cellular signaling, a protein’s glycosylation can influence the effectiveness of the drug product.

Agilent AdvanceBio Glycan Mapping provides robust HILIC separations for both UPLC and HPLC instrument platforms.

View products

Titer Determination (Affinity)

Is the protein ready to harvest? Agilent Bio-Monolith Protein A and Protein G columns deliver fast accurate quantitation of mAb titer.

View products

Titer Determination (Affinity)

Quantitation of a monoclonal antibody via affinity chromatography is used to determine when the protein is ready to harvest, or during development of optimal cell culture conditions.

Various antibodies have different affinity for either Protein A or
Protein G.

View products

New Application Compendium

What’s Inside:

  • Titer Determination
  • Intact and Subunit Purity
  • Peptide Mapping
  • Amino Acid & Cell Culture Analysis
  • Glycan Analysis
  • Aggregate & Fragment Analysis
  • Charge Variant Analysis
  • Best Practices

Download now

New Application Compendium

Download now

AdvanceBio Webinar Series

Advancing Characterization of Biotherapeutics

Presented by industry experts, this series of webinars cover the latest UHPLC techniques for the characterization of therapeutic molecules using state of the art technologies.

Access webinars

Related Stories

Be Agilent Sure in Biopharma

There has never been a better time to stream your biopharma workflows - with technology from Agilent. We offer innovative solutions every step of the way.

View solutions


For Research Use Only. Not for use in diagnostic procedures.