The Multiple Affinity Removal LC Column - Human Albumin/IgG is designed to chromatographically remove the two most interfering high-abundance proteins (albumin and IgG) from human samples (e.g., plasma, serum, urine or cerebrospinal fluid). Column is compatible with most FPLC or HPLC pumps or instruments, including the Agilent 1200/1100 instruments.
Removal of albumin and IgG improves subsequent LC/MS and electrophoretic analysis of the sample by effectively expanding the dynamic range of the analysis.
This product is not available for purchase by the general public.
Product Details
Features
Eliminates approximately 69% of total protein (albumin and IgG) when used with the Multiple Affinity Removal System
Available in a 4.6 x 50 mm format - custom sizes and spin cartridges are also available
Serum injection volume is 100 µL per run for the 4.6 mm id column
Optimize sample recovery by utilizing our rapid, single injection protocols and optimized buffers
Optimized two-buffer system allows for highly reproducible re-use of the column for over 200 injections – available in a convenient Column Reagent Starter Kit
Process up to 20,000 µL of plasma/serum over the column lifetime for the 4.6 mm id column
Protocols and columns are optimized for use with human physiological samples (not recommended for use with mouse serum)
Compatible with most FPLC or HPLC pumps or instruments, including Agilent 1200/1100 instruments
Human Albumin/IgG Affinity Removal Spin Cartridge is also available
A guide to find the right column for your characterization workflow. It includes advice and tips on method development, solvent choice, mobile phase modification, optimization, example separations, as well as ordering information.
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