Be Agilent Sure of Your Biologic's Critical Quality Attributes
The importance of understanding the attributes of a biologic drug, and the processes used to create it, cannot be underestimated.
Measure what matters
Agilent AdvanceBio columns are designed and produced to provide results you can be sure of when analyzing highly complex biotherapeutic molecules and monitoring their purity, potency, and other critical quality attributes.
Which Critical Quality Attribute are you monitoring?
Amino Acid & Cell Culture Analysis
Composition of cell culture media is critical to the quantity and quality of the biotherapeutic produced.
LC/UV with sample derivatization is a simple and robust approach to amino acid analysis.
An LC/MS-based workflow allows you to bypass sample derivatization and simultaneously detect other cell culture metabolites.
Intact & Subunit Analysis Using Reversed Phase
Biotherapeutic proteins are often heterogeneous, containing a mixture of fragments and other product-related impurities.
AdvanceBio RP mAb was specifically designed with the optimum pore size for monoclonal antibodies.
The polymer-based chemistry of PLRP-S gives excellent peak shape under formic acid conditions for MS detection.
Intact Analysis Using Hydrophobic Interaction
AdvanceBio HIC columns are designed to quickly determine post translational modifications without compromising performance.
A single chemistry is suitable for multiple applications. The chemistry is optimized to provide increased resolution and peak shape with enhanced selectivity to measure oxidation and drug to antibody ratios.
Aggregate & Fragment Analysis
Aggregation may trigger undesirable immunogenic response, making it imperative to accurately measure aggregation levels.
The inert chemistry of AdvanceBio SEC column gives excellent peak shape without mobile phase additives, ensuring the integrity of your measurement.
Charge Variant Analysis
Charge variants, especially in the complementarity determining region of mAbs, can significantly impact efficacy.
Agilent Bio mAb columns are specially designed for separation of monoclonal antibody charge variants.
Agilent Bio IEX is available in four chemistries to meet any ion exchange need.
Peptide Mapping
Peptide mapping is used to confirm primary sequence, as well as identify and quantify critical PTMs.
The AdvanceBio Peptide Mapping column offers robust performance for a wide range of proteins.
AdvanceBio Peptide Plus offers ideal peak shape under formic acid conditions for MS detection.
Glycan Analysis
As glycans are instrumental in cellular signaling, a protein’s glycosylation can influence the effectiveness of the drug product.
Agilent AdvanceBio Glycan Mapping provides robust HILIC separations for both UPLC and HPLC instrument platforms.
Titer Determination (Affinity)
Quantitation of a monoclonal antibody via affinity chromatography is used to determine when the protein is ready to harvest, or during development of optimal cell culture conditions.
Various antibodies have different affinity for either Protein A or
Protein
G.
New Application Compendium
What’s Inside:
- Titer Determination
- Intact and Subunit Purity
- Peptide Mapping
- Amino Acid & Cell Culture Analysis
- Glycan Analysis
- Aggregate & Fragment Analysis
- Charge Variant Analysis
- Best Practices
New Application Compendium
AdvanceBio Webinar Series
Advancing Characterization of Biotherapeutics
Presented by industry experts, this series of webinars cover the latest UHPLC techniques for the characterization of therapeutic molecules using state of the art technologies.
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For Research Use Only. Not for use in diagnostic procedures.