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Be Agilent Sure of Your Biologic's Critical Quality Attributes

The importance of understanding the attributes of a biologic drug, and the processes used to create it, cannot be underestimated.

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Measure what matters

Agilent AdvanceBio columns are designed and produced to provide results you can be sure of when analyzing highly complex biotherapeutic molecules and monitoring their purity, potency, and other critical quality attributes.


Which Critical Quality Attribute are you monitoring?

Aggregate and Fragment Analysis

Is our aggregation measurement accurate? Agilent AdvanceBio SEC columns deliver accurate, precise quantitation for mAb aggregation and protein analysis.

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Aggregate & Fragment Analysis

Aggregation may trigger undesirable immunogenic response, making it imperative to accurately measure aggregation levels.

The inert chemistry of AdvanceBio SEC column gives excellent peak shape without mobile phase additives, ensuring the integrity of your measurement.

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Intact and Subunit Purity

Are there impurities that we aren’t resolving? Agilent wide pore columns come in a range of chemistries to give you the resolution, selectivity, and performance needed.

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Intact & Subunit Purity

Biotherapeutic proteins are often heterogeneous, containing a mixture of fragments and other product-related impurities.

AdvanceBio RP mAb was specifically designed with the optimum pore size for monoclonal antibodies.

The polymer-based chemistry of PLRP-S gives excellent peak shape under formic acid conditions for MS detection.

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Amino Acid and Cell Culture Analysis

Is our cell culture media optimal for mAb production? Agilent AdvanceBio cell culture workflows cover all your needs.

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Amino Acid & Cell Culture Analysis

Composition of cell culture media is critical to the quantity and quality of the biotherapeutic produced.

LC/UV with sample derivatization is a simple and robust approach to amino acid analysis.

An LC/MS-based workflow allows you to bypass sample derivatization and simultaneously detect other cell culture metabolites.

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Peptide Mapping

Can we detect the PTMs that matter most? Agilent AdvanceBio columns offer complementary column choices to solve all your peptide-mapping challenges.

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Peptide Mapping

Peptide mapping is used to confirm primary sequence, as well as identify and quantify critical PTMs.

The AdvanceBio Peptide Mapping column offers robust performance for a wide range of proteins.

AdvanceBio Peptide Plus offers ideal peak shape under formic acid conditions for MS detection.

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Charge Variant Analysis

Is purification or formulation leading to protein oxidation? Agilent Bio IEX and Bio mAb columns separate the charge variants caused by a wide variety of PTMs.

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Charge Variant Analysis

Charge variants, especially in the complementarity determining region of mAbs, can significantly impact efficacy.

Agilent Bio mAb columns are specially designed for separation of monoclonal antibody charge variants.

Agilent Bio IEX is available in four chemistries to meet any ion exchange need.

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Glycan Analysis

Do we have a complete picture of our protein’s glycosylation? Agilent AdvanceBio Glycan Mapping columns are part of a complete workflow solution.

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Glycan Analysis

As glycans are instrumental in cellular signaling, a protein’s glycosylation can influence the effectiveness of the drug product.

Agilent AdvanceBio Glycan Mapping provides robust HILIC separations for both UPLC and HPLC instrument platforms.

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Titer Determination (Affinity)

Is the protein ready to harvest?

Agilent Bio-Monolith Protein A and Protein G columns deliver fast accurate quantitation
of mAb titer.

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Titer Determination (Affinity)

Quantitation of a monoclonal antibody via affinity chromatography is used to determine when the protein is ready to harvest, or during development of optimal cell culture conditions.

Various antibodies have different affinity for either Protein A or Protein G.

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AdvanceBio Webinar Series

Advancing Characterization of Biotherapeutics

Presented by industry experts, this series of webinars cover the latest UHPLC techniques for the characterization of therapeutic molecules using state of the art technologies.

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For Research Use Only. Not for use in diagnostic procedures.