6545XT AdvanceBio LC/Q-TOF
6545XT AdvanceBio LC/Q-TOF
Accurately profile major and minor proteoforms with time-of-flight technology, offering new levels of sensitivity and clarity to intact protein mass spectrometry. Easily dig deeper into peptide digests with the Iterative MS/MS mode of operation. The 6545XT AdvanceBio Q-TOF is the analytical cornerstone in a comprehensive set of tools designed for biopharma.
- Quadrupole Time of Flight LC/MS
Request a Quote
Product Details
- Get up and running fast with quick-start methods that include Agilent AdvanceBio LC columns for intact protein and peptide workflows
- Characterize both major and minor intact protein variations with an automated data workflow featuring MassHunter BioConfirm software
- Adapt your instrument to your needs with SWARM autotune to ensure optimum performance with no manual adjustment
- Large molecule tune option provides sub-nanogram detection levels for intact glycosylated monoclonal antibodies
- Be sure of your results with mass accuracies within 10 ppm for glycosylated intact proteins
- Dig deeper into your digested samples with the powerful iterative MS/MS feature
- Maximize your uptime with a system tested to perform through thousands of protein injections without degradation of performance
- Minimize maintenance delays with vent-free cleaning of entrance optics
- Integration of either MOBILion Systems’ MOBIE or e-MSion’s ExD cell is supported
| In-spectra Dynamic Range |
|
| Ion Source |
|
| MS Acquisition Rate |
|
| MS Mass Accuracy (negative) |
|
| MS Mass Accuracy (positive) |
|
| MS Sensitivity (positive) |
|
| MS/MS Acquisition Rate |
|
| MS/MS Mass Accuracy (negative) |
|
| MS/MS Mass Accuracy (positive) |
|
| MS/MS Sensitivity (positive) |
|
| Polarity Switching Duty Cycle |
|
| Quad Resolution (FWHM) |
|
| Quadrupole Isolation Mass Range |
|
| Software Platform |
|
| Supported Software Add-Ons |
|
| TOF Mass Range |
|
| TOF Mass Resolution (FWHM) |
|
| Temperature Mass Stability |
|
| Ventless Maintenance |
|
Additional technology and software capabilities for LC/Q-TOF
Agilent partners with innovators who provide technologies and software that expand the core capabilities of the LC/Q-TOF portfolio. Learn more about additional capabilities and visit the compatibility matrix to see which ones are offered for your LC/Q-TOF.
Learn More- Application Notes
-
Differentiation of Isomeric Amino Acids by Electron Capture Dissociation
Agilent 6545XT AdvanceBio LC/Q-TOF equipped with an ExD cell was used to differentiate isomeric amino acids in a tryptic peptide.
- Application Notes
- English
- 21 May 2026
- 835.33 KB
Authenticity Evaluation of Insect Protein-Containing Food Products
This note presents an LC/MS workflow for the taxonomic authentication of insect-derived ingredients in commercial cricket-based protein bars.
- Application Notes
- English
- 13 Apr 2026
- 773.90 KB
Top-Down Sequence Analysis of Intact Proteins Using an Agilent AdvanceBio 6545XT LC/Q-TOF with ExD
This application note describes a method for top-down fragmentation using the Agilent 6545XT AdvanceBio LC/Q-TOF with an electron capture dissociation (ExD) cell.
- Application Notes
- English
- 07 Jan 2026
- 790.03 KB
Streamlined Workflows for N-Glycan Analysis of Biotherapeutics with LC/FLD/MS
Streamlined workflows for N-Glycan analysis of biotherapeutics using Agilent AdvanceBio Gly-X InstantPC and 2-AB Express sample preparation with LC/FLD/MS
- Application Notes
- English
- 11 Mar 2025
- 1.19 MB
Analysis of Degradation Impurities of Glucagon-Like Peptide-1 Agonists by LC/Q-TOF MS
Characterization of forced degradation impurities of glucagon-like peptide-1 agonists by Agilent LC/Q-TOF mass spectrometry.
- Application Notes
- English
- 15 Jan 2025
- 837.39 KB
A Comparative Study of the Intact Mass, Subunit Mass, and Released Glycans of Two Rituximab Biosimilars Using High-Resolution LC/MS
A Comparative Study of the Intact Mass, Subunit Mass, and Released Glycans of Two Rituximab Biosimilars Using High-Resolution LC/MS
- Application Notes
- English
- 25 Oct 2024
- 1.15 MB
LC/MS/MS Peptide Mapping Comparison of Innovator and Biosimilars of Rituximab
The development and manufacturing of biosimilars require comparability studies with innovator biotherapeutic products.
- Application Notes
- English
- 25 Oct 2024
- 1.59 MB
Automated Monoclonal Antibody Subunit Analysis by Online Reduction Using 2D-LC/MS
This application note describes an automated 2D-LC workflow for the reduction of mAbs using Protein A affinity chromatography in the first dimension and MS-compatible size exclusion chromatography (SEC) in the second dimension.
- Application Notes
- English
- 14 Oct 2024
- 756.97 KB
LC/MS Based Characterization of GLP-1 Therapeutic Peptide Liraglutide and Impurities
LC/MS based characterization workflow of the GLP-1 therapeutic peptide liraglutide and its impurities using the 1290 Infinity II LC and 6545XT AdvanceBio LC/Q-TOF.
- Application Notes
- English
- 26 Sep 2024
- 2.78 MB
Analysis of Lipid Nanoparticle Components Using Agilent 6545XT AdvanceBio LC/Q-TOF
This application note proposes an LC/MS method for the analysis of lipid components of mRNA encapsulated in lipid nanoparticles using the 6545XT AdvanceBio LC/Q-TOF.
- Application Notes
- English
- 03 Jun 2024
- 466.49 KB
- Brochures
- Flyers
- Posters
- Technical Overviews
- White Papers
Agilent Announces Cutting-Edge Advances in GC/MS and LC/Q-TOF Technology at ASMS 2024
Agilent is introducing two new products. The Agilent 7010D Triple Quadrupole GC/MS System (7010D GC/TQ) features the new HES 2.0 ion source, providing attogram-level sensitivity, unmatched robustness, and industry-leading uptime. The Agilent ExD Cell available for the 6545XT AdvanceBio LC/Q-TOF enhances peptide and protein characterization capabilities by adding electron capture dissociation (ECD).
- 06 Jun 2024
Videos
Agilent University
Develop skills and improve lab productivity with a wide variety of learning options from Agilent Education.
- Support
-
MassHunter Data Acquisition for LC/TOF and LC/Q-TOF Familiarization Guide
This guide teaches you how to use your MassHunter Data Acquisition for Agilent LC/TOF or LC/Q-TOF system.
- Familiarization Checklists
- English
- 13 Jul 2023
- 4.44 MB
MassHunter Workstation 11.0 for LC/TOF and LC/Q-TOF Installation Guide
This book provides instructions to install the MassHunter Workstation software in these configurations: • Workstation (standalone, no content management) • Networked Workstation (networked, with content management on separate server)
- Installation Guides
- English
- 13 Jul 2023
- 967.69 KB
GC/MS and LC/MS MassHunter Workstation and Networked Workstation Requirements Guide
This document details the minimum computer hardware, software, and network requirements as well as the minimum instrument firmware revisions required to run an Agilent MassHunter Workstation or Agilent MassHunter Networked Workstation. Also included are the supported instrument types and Windows operating system configurations.
- Site Preparation Checklists
- English
- 12 Nov 2025
- 1.18 MB
- FAQs
- Quick Reference Guides
- Site Preparation Checklists
- Supplemental Information
- User Manuals