Seahorse XF Glycolytic Rate Assay Kit
The Agilent Seahorse Glycolytic Rate Assay provides a precise measure of glycolysis in live cells, revealing transient responses and rapid metabolic switches not discernible in end-point lactate assays. Run on Agilent Seahorse XF Analyzers, this assay quantifies proton efflux specific to glycolysis and should be performed using XF pH-ready media. Each kit contains the material sufficient for six full plate tests. The XF kit is for use with 96- or 24-well Seahorse Analyzers. The XFp kit is for use with 8-well Seahorse Analyzers.
This assay is not compatible with XFe96 Spheroid, XF24 V28, XF24 Islet or XF Flex 3D Capture plates.
This assay is not compatible with XFe96 Spheroid, XF24 V28, XF24 Islet or XF Flex 3D Capture plates.
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Product Details
Features
- Provides a precise measure of glycolytic rate by calculating and subtracting mitochondrial-produced acidification
- Detects metabolic switches (such as the Warburg effect) by reporting the relative activity of glycolysis and mitochondrial respiration
- Measures the effects of metabolic modulators on glycolytic rates within a single assay well
- This assay is validated and best performed with Agilent Seahorse XF DMEM medium, pH 7.4 (Cat. No. 103575-100) or Agilent Seahorse XF RPMI medium, pH 7.4 (Cat. No. 103576-100)
- The kit contains a Rotenone/Antimycin A mix to block mitochondrial activity (for calculation of mitochondrial acidification) and 2-DG (to inhibit glycolysis) as an internal control
- Single-use vials assure quality and quantity every time and are now easier to open
- Upload your assay result file to your Agilent Seahorse Analytics account to calculate and report parameters of the XF Glycolytic Rate assay, share with collaborators, or export data to Microsoft Excel or GraphPad Prism for other graphing or analysis needs
- Only XF24 V7 and XFp plate types are compatible with legacy report generators. All other plate types are not compatible with report generators.
How It Works
