Seahorse XFe24 Analyzer
Learn more about our entire cell analysis portfolio and identify the best solution for your research needs.
Learn more about How XF Technology Works
For Research Use Only. Not for use in diagnostic procedures.
- Seahorse XF Analyzers
Product Details
- Real-time Results- this integrated system reports metabolic rates in just minutes, without sample extraction or labeling. Wave software controls the instrument and performs rate measurements on the fly for same day results.
- Live-Cell Responses – detect responses to substrates, inhibitors, and other compounds in real time via the 4-port injection system and automated mixing while maintaining physiologic temperature (37oC).
- Sensitive- analyze as few as 10,000 cells per well in the custom 24-well plate. Cell number requirements vary by cell type; consult the Cell Reference Database for details. The 24-well microplate and system has capacity for larger and/or more metabolically active samples than the 96-well system.
- Compatible with a variety of samples due to the precision-controlled heating tray, which can maintain 16-42oC (12oC-20oC above ambient conditions).
- Analyze function of islet or other mobile samples with the Seahorse XF24 Islet Capture Plate
- Measure mitochondrial function with the Seahorse XF Cell Mito Stress Test
- Quantify ATP production by Glycolysis and OXPHOS simultaneously with the Seahorse XF real-time ATP rate assay
- Generate a metabolic phenotype within one hour with the Seahorse XF Cell Energy Phenotype Test
- Measure glycolytic rates in live cells with the Seahorse XF Glycolytic Rate Assay
- Quickly determine dependency of cellular energy production on mitochondrial substrates with the Seahorse XF Mito Fuel Flex Test
- Easily create assay protocols and analyze data with Seahorse Wave software
- For Research Use Only. Not for use in diagnostic procedures.
| Specification | Detail |
| Number of Assay Wells |
20-22
|
| Microchamber volume | 7 µL |
| Controller | Combination Windows 10 computer and touchscreen display with full assay design, control, and analysis capability. |
| Software | Design and analyze assay templates on Controller or Wave desktop. |
| Best For |
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| Key Advantages |
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| Plate Format | 24-well microplate |
- Compatibility with both adherent and suspension cells as well as isolated mitochondria and non-mammalian samples.
- Ability to perform up to 4 independent injections per well with automatic mixing
- Automatic calculation of oxygen consumption rate (OCR) and extracellular acidification rate (ECAR).
- Simultaneous measurement of OCR and ECAR in the same well
- Analytical Sensitivity for small sample sizes
- Label-free detection in real time
- Windows-compatible desktop analysis software (Wave) for plotting, reporting, analyzing, and exporting your XF data
- Key Literature
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Normalization of Agilent Seahorse XF Data Data by In-situ Cell Counting Using a Biotek Cytation 5
Application Note - Normalization of Agilent Seahorse XF data by in-situ cell counting using a BioTek Cytation 5
- Application Notes
- English
- 24 Mar 2017
- 931.07 KB
Seahorse XF Instrument Selection Guide
Find the perfect instrument for your analysis needs with this hepful guide.
- Selection Guides
- English
- 19 Jun 2017
- 1.05 MB
- Application Notes
- Case Studies
- Flyers
- Technical Overviews
- Utilities
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Seahorse XF Assay Guides and Templates
Seahorse XF assay guides and templates provide guidelines to quickly and confidently perform a cell line-specific XF stress test assay on your Seahorse XFe, XFp, or XF Analyzer. Once logged in, choose from a selection of assay guides or assay templates to download for your next XF stress test.
- FAQs
- Instruction Sheets
- Quick Reference Guides
- Site Preparation Checklists
- User Manuals
Videos
Beyond Lactate: Measuring Glycolytic Rates in Live Cells with the Agilent Seahorse XF Glycolytic Rate Assay
Glycolysis is a critical process in both normal cellular function and diseases such as cancer. Cells readily switch between this energy producing pathway and mitochondrial respiration, depending on energy demand and nutrient availability. As such, knowing how much glycolysis is being used by cells at any moment in time is important for understanding the interaction between metabolic activity and cell function. When glycolysis proceeds to completion, lactate and protons are extruded from the cell. The Seahorse XF Glycolytic Rate Assay Kit measures the resulting acidification rates and accounts for mitochondrial acidification to deliver the most accurate acidification-based measurement available.
Seahorse Metabolic Analyzers Enable Rapid, Quantitative Measurement of Cellular Bioenergetics
Seahorse technology is based on measuring the rate of extracellular fluxes of analytes in the media surrounding cells. Seahorse assays enable quantification of cellular bioenergetics by measuring the two energy yielding pathways, mitochondrial respiration and glycolysis, in a sensitive and convenient microplate format. A cell’s ability to respond to increased energy, biosynthetic and redox demands are in a large part influenced by two fundamental pathways, mitochondrial respiration and glycolysis.
The Hitchhiker's Guide to XF Bioenergetic Assays
XF Extracellular Flux technology is commonly used to measure cellular bioenergetics, and determine the role of metabolism in regulating cell phenotype. From measuring basic intracellular mitochondrial function, XF analysis has now evolved to employing multiple assays for the measurement of mitochondrial substrate selectivity and glycolysis. In addition XF Analyzers are also used to measure the function of isolated mitochondria, enabling the examination of mitochondria from multiple samples simultaneously, saving valuable time and resources.
Cell Reference Database - search scientific publications that reference/cite Agilent Cell Analysis products and technology by cell type, cell line, research area, assay, or author.
Publications Database - publications, in which Agilent Cell Analysis data is included, linked to PubMed.gov.
XF Instrument Selection Guide - general specifications and features of the different Seahorse Analyzer models.
Basic Procedures & Assay Protocols - guides to help users through the steps required to perform assays.
Cell Analysis Travel Awards - travel awards for researchers who present a poster or presentation citing data generated using Agilent Cell Analysis technology.
XF Analyzer Grant Expeditor Package - simplify your grant application process with the Agilent Seahorse XF Analyzer Grant Expeditor Package.
XF Core Facilities - facilities that provide access to Agilent Seahorse XF technology, including advice in experimental design, data analysis, basic training, and bioinformatics on a cost recovery basis.
Lab Instrument Service Agreements – instrument service plans that maintain instrument performance and keep laboratory operations running smoothly and consistently.