Seahorse XFe24 Analyzer
Seahorse XFe24 Analyzers measure OCR and ECAR of live cells in a 24-well plate format. OCR and ECAR rates are key indicators of mitochondrial respiration and glycolysis and these measurements provide a systems-level view of cellular metabolic function in cultured cells and ex-vivo samples.
Learn more about How XF Technology Works
Learn more about How XF Technology Works
For Research Use Only. Not for use in diagnostic procedures.
Product Details
Features
- Real-time Results- this integrated system reports metabolic rates in just minutes, without sample extraction or labeling. Wave software controls the instrument and performs rate measurements on the fly for same day results.
- Live-Cell Responses – detect responses to substrates, inhibitors, and other compounds in real time via the 4-port injection system and automated mixing while maintaining physiologic temperature (37oC).
- Sensitive- analyze as few as 10,000 cells per well in the custom 24-well plate. Cell number requirements vary by cell type; consult the Cell Reference Database for details. The 24-well microplate and system has capacity for larger and/or more metabolically active samples than the 96-well system.
- Compatible with a variety of samples due to the precision-controlled heating tray, which can maintain 16-42oC (12oC-20oC above ambient conditions).
- Analyze function of islet or other mobile samples with the Seahorse XF24 Islet Capture Plate
- Measure mitochondrial function with the Seahorse XF Cell Mito Stress Test
- Generate a metabolic phenotype within one hour with the Seahorse XF Cell Energy Phenotype Test
- Measure glycolytic rates in live cells with the Seahorse XF Glycolytic Rate Assay
- Quickly determine dependency of cellular energy production on mitochondrial substrates with the Seahorse XF Mito Fuel Flex Test
- Easily create assay protocols and analyze data with Seahorse Wave software
- For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Specification | Detail |
| Number of Assay Wells |
20-22
|
| Microchamber volume | 7 µL |
| Controller | Combination Windows 10 computer and touchscreen display with full assay design, control, and analysis capability. |
| Software | Design and analyze assay templates on Controller or Wave desktop. |
| Best For |
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| Key Advantages |
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| Plate Format | 24-well microplate |
All Agilent Seahorse XF Analyzers Feature:
- Compatibility with both adherent and suspension cells as well as isolated mitochondria and non-mammalian samples.
- Ability to perform up to 4 independent injections per well with automatic mixing
- Automatic calculation of oxygen consumption rate (OCR) and extracellular acidification rate (ECAR).
- Simultaneous measurement of OCR and ECAR in the same well
- Analytical Sensitivity for small sample sizes
- Label-free detection in real time
- Windows-compatible desktop analysis software (Wave) for plotting, reporting, analyzing, and exporting your XF data
Additional Information
XF Cell Reference Database - search scientific publications that reference/cite Agilent Seahorse XF technology by cell type, cell line, research area, XF assay, or author.
XF Publications Database - publications, in which Agilent Seahorse XF data is included, linked to PubMed.gov.
XF Instrument Selection Guide - General specifications and features of the different Seahorse Analyzer models.
Basic Procedures & Assay Protocols - guide users through the steps required to perform XF assays
XF Travel Awards - travel awards for researchers who present a poster or presentation citing data generated using Agilent Seahorse XF technology.
XF Grant Expeditor Package - simplify your grant application process with the Agilent Seahorse XF Grant Expeditor Package.
XF Travel Awards - travel awards for researchers who present a poster or presentation citing data generated using Agilent Seahorse XF technology.
XF Grant Expeditor Package - simplify your grant application process with the Agilent Seahorse XF Grant Expeditor Package.
XF Core Facilities - facilities that provide access to Agilent Seahorse XF technology, including advice in experimental design, data analysis, basic training, and bioinformatics on a cost recovery basis.
Lab Instrument Service Agreements – instrument service plans that maintain instrument performance and keep laboratory operations running smoothly and consistently.
