Hi-Plex HPLC columns provide reliable carbohydrate analysis with simple instrument requirements. Unlike ion chromatography columns, Hi-Plex columns use pure water or dilute acid on a standard HPLC, and do not require ion regeneration or suppressors. The media consists of robust a sulfonated cross-linked styrene-divinylbenzene gel in hydrogen form, or is impregnated with various metal cations with different selectivities, as described in USP L17, L19, L34, and L58. Hi-Plex HPLC columns are available in 8 μm and 10 μm particle sizes.
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Simplify the Sample Preparation Search with Intuitive eSelector Tools
Achieve better results from your analyses, while extending the lifetime of your column and instrument components. Use the eSelector Tools to quickly and easily find the right products for your sample preparation:
Simplify the HPLC Columns Search with Intuitive eSelector Tools
Agilent offers one of the broadest, most modern HPLC column portfolios with columns for nearly all separation modes and applications. Use the HPLC Column eSelector Tools to quickly and easily find the right HPLC columns. Begin selecting your HPLC column by one of the following:
Honey Composition Analysis with the Hi-Plex Column
Using high-performance liquid chromatography (HPLC) for the analysis of honey composition helps find out which sugars are present and in what percentage. The use of a Hi-Plex column is a preferred separation mechanism for the analysis of simple sugars in foodstuffs, including honey.
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Per- and polyfluoroalkyl substances (PFAS) are a large group of anthropogenic chemicals that have been applied in industrial, commercial, and domestic products since the 1950s. Because of their toxicity, persistence, and bioaccumulation potential, PFAS have become global environmental pollutants. Besides the environment, the food chain represents another source of exposure, and the risk related to the presence of PFAS in foods has become of increased interest. Whole milk, infant formula, and ingredients used in infant formula production represent important foodstuffs that require sensitive methods with reporting limits at low parts per billion levels or lower for multiple PFAS. This article summarizes optimization experiments and validation of a complete workflow, including sample preparation and a liquid chromatography–tandem mass spectrometry (LC–MS/MS) method for determination of sixteen priority PFAS analytes listed by the U.S. Food and Drug Administration (FDA).