![pCFB-EGSH Retroviral Vector pCFB-EGSH Retroviral Vector](/cs/publishingimages/Black box 1-320x320.jpg)
pCFB-EGSH Retroviral Vector
A limitation to the use of plasmid-based vectors for controlled gene expression is the fact that many cell types of academic, industrial or clinical interest are difficult or virtually impossible to transfect using current transfection methods. In particular, primary human cell lines, lymphocytes, neurons and other nondividing cells are best transduced using viral delivery systems. The most popular and user-friendly of these are the retroviral vectors, like pCFB-EGSH. Infection with retroviruses often yields transduction efficiencies close to 100%, and the proviral copy number can be easily controlled by varying the multiplicity of infection (MOI).