pDual Expression System
The pDual GC Vector offers a C-terminal tag with three copies of the human c-Myc epitope tag (one copy is EQKLISEEDL) and a single copy of the 6xHis purification tag. The c-Myc epitope and 6xHis purification tags, expressed on the C-terminus of the protein, can be used for easy detection and purification of the fusion protein from mammalian and bacterial cell lysates. Use of the c-Myc epitope and the 6xHis purification tags allows every target gene to be detected and purified using the exact same protocol and reagents, saving you time and money.
For constitutive expression in mammalian cells, the pDual expression vector contains a mutagenized version of the CMV promoter/enhancer. Inducible gene expression in prokaryotes is directed from the hybrid T7/lacO promoter.