The MBS Mammalian Transfection Kit increases transfection efficiencies by up to 100-fold. Cells are incubated with CaPO4/DNA coprecipitate for only 3 hours compared to 6 to 12 hours with traditional CaPO4 methods. Cells can be assayed 12 to 24 hours post-transfection instead of the typical 48 to 72 hours. This method is particularly suited for the generation of transient transfectants due to the short incubation time required.
However, stable transfectants have also been generated with increased efficiency.
For Research Use Only. Not for use in diagnostic procedures.