
ArcticExpress (DE3) Competent Cells
Forced high-level expression of a heterologous protein in E. coli can result in the production of large amounts of incorrectly folded protein, generating aggregates of inactive protein known as inclusion bodies. While the aggregated protein may be easy to purify, obtaining active protein from inclusion bodies typically requires protein-specific and labor-intensive in vitro re-folding steps, with no guarantee of obtaining biologically active product. ArcticExpress (D3) competent cells provide an in vivo approach to increasing the yield of soluble protein produced in E. coli.