SureSelect Max DNA Library Prep Kit

RUO

The SureSelect Max DNA library prep kit features robust performance and streamlined protocols, optimized for a range of input amounts and sample types, including FFPE and genomic DNA. The modular portfolio, with standalone reagent components, enables a true one-day workflow from sample to enriched library, supporting both enzymatic and mechanical shearing methods.

Achieve optimal performance for reduced costs with a fully modularized portfolio of library prep and enrichment reagents for DNA sequencing. Obtain more actionable data with full compatibility with SureSelect custom and catalog probes. Seamless integration of Agilent sample quality control and automation solutions enhance productivity with complete integration into your NGS workflow.

DNA-Seq Reagents

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Features

Deeper and more uniform coverage across targeted regions reduces sequencing costs
Ability to generate high complexity libraries with as little as 10 ng of DNA input from intact or highly fragmented FFPE DNA
True one-day prep enables shorter turnaround time from samples to enriched libraries, including streamlined protocols to reduce the chance of user error
Fully modular portfolio of reagents offers unparalleled workflow and application flexibility, including standalone purification beads and adapters, primers, and blockers for some non-Illumina platforms
Optimized pre-capture pooling performance and enhanced yield for enrichment with minimal PCR cycling reduce sequencing costs and minimize user error during hybridization
Single/duplex molecular barcodes (MBC) remove false positives and accurately detect variants down to ≤ 1% variant allele frequency, with optional MBC-free adaptors for germline applications
384 unique dual indexes minimize index hopping
Automation improves lab efficiency, including the Agilent Bravo NGS workstation that allows for scaling up to 96 samples per run
One easy, parallel workflow for targeted RNA and DNA sequencing using SureSelect Max chemistry simplifies sample processing
Protocol support for whole genome sequencing (WGS) with low-cycle PCR

Specifications


Automation Compatible	Yes
Hybridization Time	90-minute (Fast Hyb) 16-24 hrs (Overnight Hyb)
Multiplexity	384 Unique dual indexes
Sample Input	10-200 ng
Tissue Sample Type	FFPE Fresh Frozen Tissue Blood
Turnaround Time	<8 hr (fast hyb) 2 days (overnight hyb)

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SureSelect Max DNA Library Prep Kit

Product Details

Agilent SureSelect Max DNA Library Preparation and Target Enrichment Kits Data Sheet

SureSelect Max Index Sequence Resource Excel Spreadsheet

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