Economical Multimode Microplate Reader, BioTek Synergy HTX

Features

Flexibility at an affordable price in a high-performance instrument. The hardware design and Gen6 software drive functionality and address a range of multimode applications without costly and often unused options
The combination of independent optics allows multimode measurements in a single instrument. Great sensitivity with filter-based fluorescence optics and monochromator-based absorbance optics for filter-free UV-Vis measurements
Environmental controls and robust shaking profiles enable consistent data in live cell assay workflows, including temperature control to 50 °C
Excellent Alpha assay performance with sensitive fluorescence. Enhanced performance for many applications including important AlphaScreen and AlphaLISA assays
Fast inject/read assays use the available dual reagent injector module with angled tips. This operation delivers results for enzyme kinetics, dual luciferase reporter assays, and many others. Simplify your workflow and save time in your critical assays
Microvolume nucleic acid and protein quantification with the Take3 microvolume plate

Specifications


Automation Compatibility	BioStack 3rd-party
Environment Control	4-Zone Incubation to 50 °C Condensation Control
Filter Detection Modes	Fluorescence Intensity Alpha Luminescence
Imager/Reader Options	Reagent injectors
Instrument Control	Computer Software
Monochromator Detection Modes	UV-Vis Absorbance Time Resolved Fluorescence (secondary)
Shaking	Linear Orbital
Supported Labware	6 to 384-well plates Take3/Take3 Trio PCR plates Cell culture dishes

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How It Works

Great flexibility at a low price

Affordability does not mean compromising on performance with the Synergy HTX. The hardware design and Gen6 software-driven functionality address a wide range of multimode applications without costly and often unused options.

Monochromator and filter optics

Synergy HTX provides great sensitivity with its filter-based fluorescence optics, and the monochromator-based UV-Vis absorbance optics offers 200 to 999 nm measurements without requiring filters. The combination of independent optics allows multimode measurements without compromise.

Shaking for assay optimization

Assays that require shaking, such as bacterial and yeast growth studies, will benefit from the robust, programmable shaking options in Synergy HTX. Select linear or orbital shaking, with programmable speed and duration.

Applications

Excellent Alpha assay performance

The sensitive fluorescence optics in Synergy HTX provide great performance for many applications including important AlphaScreen and AlphaLISA assays.

Learn about Alpha detection technology

Fast inject/read assays

The available dual reagent injector module with angled tips, enables fast inject/read operations, which are critical for enzyme kinetics, Dual-Luciferase Reporter and many other assays. Synergy HTX is easily upgradable to add the dual reagent injector.

View applications

Microvolume measurements with the Take3 Plate

The Take3 microvolume plates expand Epoch 2 capabilities to measure 2 µL samples for direct DNA, RNA, and protein quantification. Get rapid results for up to 48 samples at a time without additional instrumentation.

Learn more about Take3 & Take3 Trio Microvolume Plates

Technology

Alpha detection

AlphaScreen is a bead-based assay technology used to study biomolecular interactions in a microplate format. The acronym 'Alpha' stands for amplified luminescent proximity homogeneous assay. This platform provides a nonradioactive, homogeneous assay that has low background and high signal to background ratios.

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Absorbance

Absorbance refers to the ability of a substance to absorb light of a specific wavelength. In a microplate reader designed to measure absorbance, a light source illuminates the sample using a specific wavelength, selected by an optical filter or a monochromator.

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